Intranuclear actin bundles induced by dimethyl sulfoxide in interphase nucleus of Dictyostelium.
Intranuclear actin bundles induced by dimethyl sulfoxide in interphase nucleus of Dictyostelium.
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DOI：
10.1083/jcb.76.1.146
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发表时间：
1978-01
期刊：
影响因子：
--
通讯作者：
Fukui Y
中科院分区：
文献类型：
--
作者：
Fukui Y
电子显微镜证据表明，二甲基亚砜 (DMSO) 会诱导细胞粘菌盘基网柄菌的间期核中形成巨大的核内微丝束。这些巨束大约长3微米，宽0.85微米，由直径6纳米的微丝组成。使用甘油细胞的研究表明，这些微丝结合兔骨骼肌重部分肌球蛋白，形成典型的装饰“箭头”结构，并且这种结合可以通过三磷酸镁腺苷恢复。这些数据证实核内微丝是收缩蛋白肌动蛋白，并且DMSO影响核内肌动蛋白，诱导这种巨大束的形成。用 DMSO 处理后，两种不同种类的细胞粘霉菌中的任何发育阶段都会出现核内肌动蛋白束。讨论了核内肌动蛋白分子的天然形式及其可能的功能，并提出收缩蛋白在细胞核中具有重要功能。
Electron microscopic evidence demonstrated that dimethyl sulfoxide (DMSO) induces formation of giant intranuclear microfilament bundles in the interphase nucleus of a cellular slime mold, Dictyostelium. These giant bundles are approximately giant bundles are approximately 3 micrometer long, 0.85 micrometer wide, and composed of microfilaments 6 nm in diameter. Studies in which glycerinated cells were used showed that these microfilaments bind rabbit skeletal muscle heavy meromyosin, forming typical decorated "arrowhead" structures, and that this binding can be reverted by Mg-adenosine triphosphate. These data verify that the intranuclear microfilaments are the contractile protein actin, and that DMSO affects intranuclear actin, inducing the formation of such giant bundles. The intranuclear actin bundles appear at any developmental stage in two different species of cellular slime molds after treatment with DMSO. The native form of the intranuclear actin molecules and their possible functions are discussed, and it is proposed that the contractile protein has essential functions in the cell nucleus.