Genotoxicity/mutagenicity of formaldehyde revealed by the Arabidopsis thaliana plants transgenic for homologous recombination substrates
Genotoxicity/mutagenicity of formaldehyde revealed by the Arabidopsis thaliana plants transgenic for homologous recombination substrates
复制标题
同源重组底物转基因拟南芥植物揭示的甲醛的遗传毒性/致突变性
DOI：
10.1016/j.mrgentox.2010.04.009
复制
发表时间：
2010-06-17
影响因子：
1.9
通讯作者：
Yu, Zengliang
中科院分区：
文献类型：
--
作者：
Li, Fanghua;Liu, Ping;Yu, Zengliang
甲醛（FA）是一种主要的工业化学品，广泛用于合成树脂和化学品的生产。在日常生活中，含FA的工业材料的使用使人类广泛接触FA。大量研究表明，FA具有遗传毒性，在体内外均可引起多种遗传毒性效应。FA引起的DNA损伤主要是DNA-蛋白质交联（DPC）。近年来，有研究表明同源重组（HR）机制参与了DPC的修复，提示同源重组可能是FA遗传毒性/致突变性的潜在指标。然而，目前还没有报道携带重组底物的微生物用于检测FA的遗传毒性/致突变作用。在本研究中。利用转GUS重组底物基因的拟南芥（Arabidopsis thaliana）品系研究了FA的遗传毒性/致突变性，结果表明，FA暴露显著增加了生长植物中HR的诱导，但对休眠种子没有影响。我们还观察到，在FA暴露后，HR相关基因AtRAD 54的表达早期上调。此外，谷胱甘肽（GSH）的预处理大大抑制了由FA暴露引起的HR诱导。(C)2010爱思唯尔有限公司版权所有。
Formaldehyde (FA) is a major industrial chemical and has been extensively used in the manufacture of synthetic resins and chemicals. The use of FA-containing industrial materials in daily life exposes human to FA extensively. Numerous studies indicate that FA is genotoxic, and can induce various genotoxic effects in vitro and in vivo. The primary DNA lesions induced by FA are DNA-protein crosslinks (DPCs). Recently, it has been reported that the homologous recombination (HR) mechanism is involved in the repair of DPCs, suggesting the homologous recombination could be a potential indicator for the genotoxicity/mutagenicity of FA. However, it has not yet been reported that organisms harboring recombination substrates are used for the detection of genotoxic/mutagenic effects of FA. In this present study. an Arabidopsis thaliana-line transgenic for GUS recombination substrates was used to study the genotoxicity/mutagenicity of FA, and the results showed that FA-exposure significantly increased the induction of HR in growing plants, but not in dormant seeds. We also observed an early up-regulation of expression of HR-related gene, AtRAD54, after FA-exposure. Moreover, the pretreatment with glutathione (GSH) suppressed drastically the induction of HR by FA-exposure. (C) 2010 Elsevier B.V. All rights reserved.