Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats.
Effect of Sex and Cross-Sex Hormone Treatment on Renal Monocarboxylate-Transporter Expression in Rats.
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DOI：
10.3390/pharmaceutics15102404
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发表时间：
2023-09-29
期刊：
影响因子：
5.4
通讯作者：
Felmlee MA
中科院分区：
文献类型：
--
作者：
Wei H;Lee A;Zhang Q;Felmlee MA
质子和钠依赖的单羧酸转运体（mct /SMCTs）是通过肾重吸收单羧酸底物来清除肾脏的决定因素。先前对完整雌鼠和雄鼠、去势雌鼠和去势雄鼠的研究发现，激素对肾单羧酸转运蛋白表达的调节，促使人们对个体激素的调节作用进行研究。本研究旨在探讨外源性性激素和异性激素对肾脏MCT1、MCT4、CD147和SMCT1 mRNA及膜结合蛋白表达的影响。卵巢切除（OVX）雌性和阉割（CST）雄性Sprague Dawley大鼠在采集肾脏前接受雌激素和/或黄体酮、睾酮或相应的安慰剂治疗21天。采用qPCR和Western blot方法定量检测mRNA和膜蛋白水平。定量分析显示，雌激素/孕激素联合治疗降低了MCT1和4的膜表达，增加了SMCT1的表达，而睾酮治疗增加了MCT1膜蛋白的表达。相关性分析显示血浆17β-雌二醇与MCT1、MCT4膜表达呈负相关，而睾酮与MCT1、MCT4膜表达呈正相关。SMCT1膜表达与17β-雌二醇和黄体酮浓度呈正相关。OVX和CST大鼠在接受性激素和异性激素治疗后，MCT1、MCT4、CD147和SMCT1的肾脏表达对雌性和雄性性激素的响应发生了显著改变。性激素、性激素受体的复杂作用以及青春期对MCT/SMCT调控的影响需要进一步的研究。
Proton- and sodium-dependent monocarboxylate transporters (MCTs/SMCTs) are determinants of renal clearance through the renal reabsorption of monocarboxylate substrates. Prior studies with intact females and males, ovariectomized females and castrated males have revealed the hormonal regulation of renal monocarboxylate-transporter expression, prompting investigation into the regulatory role of individual hormones. The aim of the present study is to evaluate the effect of exogenous sex and cross-sex hormones on renal MCT1, MCT4, CD147 and SMCT1 mRNA and membrane-bound protein expression. Ovariectomized (OVX) females and castrated (CST) male Sprague Dawley rats received estrogen and/or progesterone, testosterone, or a corresponding placebo treatment for 21 days prior to kidney collection. The quantitative measurement of mRNA and membrane-protein levels were conducted using qPCR and Western blot. Quantitative analysis revealed the combination estrogen/progesterone treatment reduced membrane MCT1 and 4 expression and increased SMCT1 expression, while testosterone administration increased MCT1 membrane-protein expression. Correlation analysis indicated that plasma 17β-estradiol was negatively correlated with MCT1 and MCT4 membrane expression, while testosterone was positively correlated. In contrast, SMCT1 membrane expression was positively correlated with 17β-estradiol and progesterone concentrations. MCT1, MCT4, CD147 and SMCT1 renal expression are significantly altered in response to female and male sex hormones following sex and cross-sex hormone treatment in OVX and CST rats. Further studies are needed to understand the complex role of sex hormones, sex hormone receptors and the impact of puberty on MCT/SMCT regulation.
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影响因子：
3.4
作者：
Gopal, E.;Umapathy, N. S.;Prasad, P. D.
通讯作者：
Prasad, P. D.
影响因子：
--
作者：
Manente, Arcangela G.;Pinton, Giulia;Moro, Laura
通讯作者：
Moro, Laura
影响因子：
7.9
作者：
Hutson, Dillion D.;Gurrala, Rakesh;Lindsey, Sarah H.
通讯作者：
Lindsey, Sarah H.
影响因子：
5.2
作者：
Brundin PMA;Landgren BM;Fjällström P;Shamekh MM;Gustafsson JÅ;Johansson AF;Nalvarte I
通讯作者：
Nalvarte I
影响因子：
3.6
作者：
Boussouar, F;Mauduit, C;Benahmed, M
通讯作者：
Benahmed, M