Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme
Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme
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表达氯病毒酶的培养烟草细胞 (BY-2) 中透明质酸的合成
DOI：
10.1002/bit.24783
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and T. Yamada
中科院分区：
文献类型：
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作者：
Rakkhumkaew;N.;S. Shibatani;T. Kawasaki;M. Fujie;and T. Yamada
从动物或微生物发酵中提取透明质酸有被病原体和微生物毒素污染的风险。在这项工作中，成功地将氯病毒透明质酸合成酶(CvHAS)基因转化烟草培养细胞(BY-2)生产透明质酸。细胞学研究表明，透明质酸在细胞上积累，也在亚细胞组分(原生质体、小质体、液泡体和液泡)中积累。携带vSPO-cvHAS结构的转基因BY-2细胞含有连接到cvHAS N端的空泡靶向信号，在空泡中积累了大量的HA。这些结果表明，cvHAS成功地在液泡膜上发挥作用，合成/转运HA进入液泡。利用该体系高效合成透明质酸为实际生产提供了一种新的方法。生物技术。比昂斯。2013；110：1174-1179。©2012 Wiley期刊，Inc.
Extraction of hyaluronan from animals or microbial fermentation has risks including contamination with pathogens and microbial toxins. In this work, tobacco cultured‐cells (BY‐2) were successfully transformed with a chloroviral hyaluronan synthase (cvHAS) gene to produce hyaluronan. Cytological studies revealed accumulation of HA on the cells, and also in subcellular fractions (protoplasts, miniplasts, vacuoplasts, and vacuoles). Transgenic BY‐2 cells harboring a vSPO‐cvHAS construct containing the vacuolar targeting signal of sporamin connected to the N‐terminus of cvHAS accumulated significant amounts of HA in vacuoles. These results suggested that cvHAS successfully functions on the vacuolar membrane and synthesizes/transports HA into vacuoles. Efficient synthesis of HA using this system provides a new method for practical production of HA. Biotechnol. Bioeng. 2013; 110: 1174–1179. © 2012 Wiley Periodicals, Inc.