MicroRNA-30e-5p promotes cell growth by targeting PTPN13 and indicates poor survival and recurrence in lung adenocarcinoma.
MicroRNA-30e-5p promotes cell growth by targeting PTPN13 and indicates poor survival and recurrence in lung adenocarcinoma.
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DOI：
10.1111/jcmm.13198
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发表时间：
2017-11
影响因子：
5.3
通讯作者：
Li GF
中科院分区：
文献类型：
--
作者：
Zhuang L;Shou T;Li K;Gao CL;Duan LC;Fang LZ;Zhang QY;Chen ZN;Zhang C;Yang ST;Li GF
异常的microRNA表达参与各种细胞过程的调节，例如包括癌症在内的多种疾病中的增殖和转移。MicroRNA-30 e-5 p（miR-30 e）以前被报道为某些恶性肿瘤中的致癌或肿瘤抑制miRNA，但其在肺腺癌（LAC）中的功能仍不清楚。在这项研究中，我们发现miR-30 e在LAC组织和细胞系中的表达增加，与肿瘤大小相关，并代表LAC患者总生存期和复发的独立预后因素。进一步的功能实验表明，miR-30 e的敲低抑制细胞生长，而其过表达促进体外和体内LAC细胞和异种移植物的生长。从机制上讲，PTPN 13被鉴定为LAC中miR-30 e的直接靶点，其中PTPN 13在LAC组织中表达下调，并与miR-30 e表达呈负相关。PTPN 13的过表达抑制了细胞生长，并通过抑制EGFR信号传导挽救了miR-30 e的促增殖作用。总之，我们的研究结果表明，miR-30 e可以通过靶向PTPN 13在LAC中作为癌基因发挥作用，并作为治疗LAC的潜在治疗靶点。
Aberrant microRNA expression is involved in the regulation of various cellular processes, such as proliferation and metastasis in multiple diseases including cancers. MicroRNA‐30e‐5p (miR‐30e) was previously reported as an oncogenic or tumour suppressing miRNA in some malignancies, but its function in lung adenocarcinoma (LAC) remains largely undefined. In this study, we found that the expression of miR‐30e was increased in LAC tissues and cell lines, associated with tumour size and represented an independent prognostic factor for overall survival and recurrence of LAC patients. Further functional experiments showed that knockdown of miR‐30e suppressed cell growth while its overexpression promoted growth of LAC cells and xenografts in vitro and in vivo. Mechanistically, PTPN13 was identified as the direct target of miR‐30e in LAC, in which PTPN13 expression was down‐regulated in LAC tissues and showed the inverse correlation with miR‐30e expression. Overexpression of PTPN13 inhibited cell growth and rescued the proliferation‐promoting effect of miR‐30e through inhibition of the EGFR signalling. Altogether, our findings suggest that miR‐30e could function as an oncogene in LAC via targeting PTPN13 and act as a potential therapeutic target for treating LAC.
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影响因子：
4.6
作者：
Liu YF;Spinelli A;Sun LY;Jiang M;Singer DV;Ginnan R;Saddouk FZ;Van Riper D;Singer HA
通讯作者：
Singer HA
影响因子：
--
作者：
Cinegaglia NC;Andrade SC;Tokar T;Pinheiro M;Severino FE;Oliveira RA;Hasimoto EN;Cataneo DC;Cataneo AJ;Defaveri J;Souza CP;Marques MM;Carvalho RF;Coutinho LL;Gross JL;Rogatto SR;Lam WL;Jurisica I;Reis PP
通讯作者：
Reis PP
miR-483-5p通过靶向RhoGDI1和ALCAM促进肺腺癌的侵袭和转移
影响因子：
11.2
作者：
Song, Qiancheng;Xu, Yuanfei;Bai, Xiaochun
通讯作者：
Bai, Xiaochun
影响因子：
15.9
作者：
Jiang, Lili;Lin, Chuyong;Li, Mengfeng
通讯作者：
Li, Mengfeng
影响因子：
56.9
作者：
Wang, ZH;Shen, D;Velculescu, VE
通讯作者：
Velculescu, VE