Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes
Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes
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DOI：
10.1002/j.1460-2075.1996.tb00953.x
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发表时间：
1996-10-15
期刊：
影响因子：
11.4
通讯作者：
Somssich, IE
中科院分区：
文献类型：
--
作者：
Rushton, PJ;Torres, JT;Somssich, IE
PR 1是欧芹基因组中由三个基因组成的家族编码的一种病程相关蛋白在瞬时表达系统中的功能丧失和获得实验证明在PR 1 -1和PR 1 -2启动子中的每一个中存在两个真菌诱导子响应元件。这些元件W1、W2和W3，含有序列（T）TGAC（C）和破坏该序列的突变使功能丧失，凝胶迁移实验证明W1、W2和W3与类似的核蛋白特异性结合，通过South-Western筛选分离出三个编码序列特异性DNA结合蛋白的cDNA克隆，并将这些蛋白命名为WRKY 1，2和3所示的氨基酸序列也特异性结合W1、W2和W3。WRKY 1、2和3是序列特异性DNA结合蛋白家族的成员，我们称之为WRKY家族。用特异性寡肽激发子Pep 25处理欧芹细胞诱导WRKY 1和3的mRNA水平的瞬时和极快的增加，相反，WRKY 2 mRNA水平显示伴随的瞬时降低，WRKY mRNA水平响应于确定的信号分子的这些快速变化表明WRKY 1、2和3在导致从激发子感知到PR 1基因激活的信号转导途径中起关键作用。
PR1 is a pathogenesis-related protein encoded in the parsley genome by a family of three genes (PR1-1, PR1-2 and PR1-3), Loss- and gain-of-function experiments in a transient expression system demonstrated the presence of two fungal elicitor responsive elements in each of the PR1-1 and PR1-2 promoters, These elements, W1, W2 and W3, contain the sequence (T)TGAC(C) and mutations that disrupt this sequence abolish function, Gel shift experiments demonstrated that W1, W2 and W3 are bound specifically by similar nuclear proteins, Three cDNA clones encoding sequence-specific DNA-binding proteins were isolated by South-Western screening and these proteins, designated WRKY1, 2 and 3, also bind specifically to W1, W2 and W3. WRKY1, 2 and 3 are members of the family of sequence-specific DNA-binding proteins, which we call the WRKY family, Treatment of parsley cells with the specific oligopeptide elicitor Pep25 induced a transient and extremely rapid increase in mRNA levels of WRKY1 and 3, WRKY2 mRNA levels in contrast showed a concomitant transient decrease, These rapid changes in WRKY mRNA levels in response to a defined signal molecule suggest that WRKY1, 2 and 3 play a key role in a signal transduction pathway that leads from elicitor perception to PR1 gene activation.