Activation of protease by sol-gel entrapment into organically modified hybrid silicates
Activation of protease by sol-gel entrapment into organically modified hybrid silicates
复制标题
通过溶胶-凝胶包埋到有机改性杂化硅酸盐中激活蛋白酶
DOI：
10.1023/a:1013876313806
复制
发表时间：
2004
影响因子：
2.7
通讯作者：
K. Kawakami
中科院分区：
文献类型：
--
作者：
S. Furukawa;T. Ono;H. Ijima;K. Kawakami
为制备一种在有机介质中对丁酸乙烯酯与3-甲基-1-丁醇（异戊醇）酯交换反应具有高活性的固定化蛋白酶，采用溶胶-凝胶法将蛋白酶包埋在有机-无机杂化硅胶Celite 545上。当丙基三甲氧基硅烷与四甲氧基硅烷以16：1的摩尔比混合作为有机硅烷前体时，在35至85 °C下，在Celite 545上的杂化凝胶包埋的蛋白酶具有8倍于在Celite 545上沉积的蛋白酶的活性。
To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methyl-1-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic–inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85 °C.