Stratification of Archaeal communities in shallow sediments of the Pearl River Estuary, Southern China
Stratification of Archaeal communities in shallow sediments of the Pearl River Estuary, Southern China
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中国南方珠江口浅层沉积物中古菌群落的分层
DOI：
10.1007/s10482-011-9548-3
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发表时间：
2011-05-01
影响因子：
2.6
通讯作者：
Wang, Fengping
中科院分区：
文献类型：
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作者：
Jiang, Lijing;Zheng, Yanping;Wang, Fengping
众所周知，微生物在沿海环境中的碳的地球化学循环中发挥着重要作用。为了解珠江口沉积物中古菌群落的组成及其生态作用，首次报道了珠江口沉积物柱状样中古菌的16 SrRNA和mcrA基因的多样性和垂直分布。定量PCR分析表明，沉积物中古菌的数量为106 ~ 107 16 S rRNA基因拷贝/g（湿重），mcrA/16 S rRNA基因拷贝的比例为11%~ 45%。构建并分析了表层（0-6 cm）、中层（18-24 cm）、硫酸盐-甲烷过渡区（SMTZ，32-42 cm）和底层（44-50 cm）沉积物的16 S rRNA基因库。结果表明，沉积物中的主要成分为杂泉古菌群（MCG）。MCG古菌可进一步分为MCG-A、B、C、D、E和F 6个亚群。另一方面，在所有沉积物层中检测到与甲烷微生物目和ANME-2甲烷氧化菌相关的产甲烷菌的mcrA序列。综上所述，我们的数据揭示了一个在很大程度上未知的古菌群落，其中MCG占主导地位的珠江河口沉积物，而甲烷菌和甲烷氧化古菌参与甲烷代谢，也被发现在社区。这是阐明珠江口古菌生物地球化学作用的第一步。
Microorganisms are known to play fundamental roles in the biogeochemical cycling of carbon in the coastal environments. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of archaea in a sediment core was reported based on the 16S rRNA andmcrAgenes for the first time. Quantitative PCR analysis revealed that archaea were present at 106–10716S rRNA gene copies/g (wet weight) in the sediment core, and the proportion ofmcrAversus 16S rRNA gene copies varied from 11 to 45%. 16S rRNA gene libraries were constructed and analyzed for the top layer (0–6 cm), middle layer (18–24 cm), sulfate-methane transition zone (SMTZ, 32–42 cm), and bottom layer (44–50 cm) sediments. The results indicated that Miscellaneous Crenarchaeotal Group (MCG) was the main component in the sediments. The MCG archaea could be further divided into six subgroups: MCG-A, B, C, D, E, and F. On the other hand,mcrAsequences from methanogens related to the orderMethanomicrobialesand ANME-2 methanotrophs were detected in all sediment layers. Taken together, our data revealed a largely unknown archaeal community in which MCG dominated within the Pearl River estuarine sediments, while methanogens and methane-oxidizing archaea putatively involving in methane metabolism, were also found in the community. This is the first important step towards elucidating the biogeochemical roles of these archaea in the Pearl River Estuary.