Polydopamine nanoparticles-assisted impedimetric sensor towards label-free lung cancer cell detection
Polydopamine nanoparticles-assisted impedimetric sensor towards label-free lung cancer cell detection
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DOI：
10.1016/j.msec.2020.111549
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发表时间：
2021-02-01
影响因子：
7.9
通讯作者：
Abaci, Serdar
中科院分区：
文献类型：
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作者：
Bolat, Gulcin;Vural, Oznur Akbal;Abaci, Serdar
聚多巴胺纳米颗粒（PDA NPs），在自然灵感的建筑材料，显示特殊的功能，用于生物医学系统和探索PDA衍生的纳米结构的未来发展是一个快速增长的领域。在此，我们证明了PDA纳米颗粒用于肺癌细胞电化学测定的第一次评估。在本研究中，PDA纳米颗粒合成在一个温和的和具有成本效益的方式，多巴胺在碱性环境中的自聚合。结构和化学表征清楚地证明了具有可控尺寸（130 nm）的PDA NPs的形成，因此作为合适的材料应用于功能化铅笔石墨电极（PGE）表面以构建细胞传感纳米探针。研究了所开发的传感器（PDA NPs/PGE）用于无标记电化学检测A-549肺癌细胞的能力。该传感器具有良好的生物相容性和灵敏度，对A-549细胞的阻抗检测线性范围较宽（1.0 × 10（2）~ 1.0 × 10（5）cells mL（-1）），检测限较低（25 cells mL（-1））。此外，所开发的生物测定法作为液体活检用于早期癌症检测具有很大的潜力。
Polydopamine nanoparticles (PDA NPs), among nature-inspired building materials, show special functions for biomedical systems and exploring PDA derived nanostructures for future developments is a fast growing field. Herein, we demonstrated the first evaluation of the PDA NPs for the electrochemical determination of lung cancer cells. In the presented study, PDA NPs were synthesized in a mild and cost-effective fashion by self-polymerization of dopamine in an alkaline environment. The structural and chemical characterizations clearly demonstrated the formation of PDA NPs with controllable size (130 nm), hence applied as a suitable material to functionalize the pencil graphite electrode (PGE) surface to construct a cytosensing nanoprobe. The ability of the developed sensor (PDA NPs/PGE) for label-free electrochemical A-549 lung cancer cells detection was investigated. The designed PDA NPs based cytosensor exhibited good biocompatibility and sensitivity for impedimetric diagnosis of A-549 cells in a wide linear range (1.0 x 10(2)-1.0 x 10(5) cells mL(-1)) with low detection limit (25 cells mL(-1)). Furthermore, the developed bioassay has great potential as liquid biopsy for early cancer detection.