Who can cleave DMSP?
Who can cleave DMSP?
复制标题
DOI：
10.1126/science.aac5661
复制
发表时间：
2015-06
期刊：
影响因子：
56.9
通讯作者：
A. Johnston
中科院分区：
文献类型：
--
作者：
A. Johnston
来自丰富的海洋真核生物的DMSP裂解酶与已知的细菌酶根本不同。[Also见Alcolombri等人的报告]海洋生物通过产生二甲基硫（DMS）在全球硫循环中发挥关键作用，二甲基硫是一种挥发性化合物，被排放到大气中。在本期的第1466页，Alcolombri等人（1）报告了丰富的海洋浮游植物Emiliania huxleyi（见图片）如何从二甲基磺基丙酸酯（DMSP）中产生DMS。使用一系列经典的生物化学方法，通过基因组和蛋白质组学分析，作者分离出了将DMSP切割成丙烯酸酯和DMS的酶和相应的基因（称为Alma1）。他们还在甲藻中发现了一种功能性的Alma1样酶，这是一种非常不同类型的丰富的单细胞海洋浮游生物，强调了这种新发现的DMSP裂解酶的广泛重要性。
A DMSP lyase from an abundant marine eukaryote differs fundamentally from known bacterial enzymes. [Also see Report by Alcolombri et al.] Marine organisms play a key role in the global sulfur cycle by producing dimethyl sulfide (DMS), a volatile compound that is emitted into the atmosphere. On page 1466 of this issue, Alcolombri et al. (1) report how the abundant marine phytoplankton Emiliania huxleyi (see the image) produces DMS from dimethylsulfoniopropionate (DMSP). Using a series of classical biochemical approaches, augmented by genomic and proteomic analyses, the authors isolated the enzyme and corresponding gene (termed Alma1) that cleaves DMSP into acrylate and DMS. They also found a functional Alma1-like enzyme in a dinoflagellate, a very different type of abundant single-cell marine plankton, emphasizing the widespread importance of this newly discovered DMSP lyase.