Exogenous chlorogenic acid alleviates oxidative stress in apple leaves by enhancing antioxidant capacity
Exogenous chlorogenic acid alleviates oxidative stress in apple leaves by enhancing antioxidant capacity
复制标题
外源绿原酸通过增强抗氧化能力减轻苹果叶片的氧化应激
DOI：
10.1016/j.scienta.2020.109676
复制
发表时间：
2020-12-15
影响因子：
4.3
通讯作者：
Lyu, Deguo
中科院分区：
文献类型：
--
作者：
Mei, Yimeng;Sun, Huili;Lyu, Deguo
被认为是一种抗氧化剂，绿原酸参与抵抗昆虫和病原体，并对人类健康产生有益影响。然而，关于绿原酸在植物中抗氧化胁迫的抗氧化功能知之甚少。以苹果离体叶片为材料，研究外源绿原酸对甲基紫精介导的氧化胁迫的缓解作用。结果表明，外源绿原酸能有效缓解叶绿素含量和最大潜在光系统II效率的下降。外源绿原酸还有效地降低膜损伤和脂质氧化，并刺激抗氧化酶，包括过氧化物酶，过氧化氢酶，多酚氧化酶的活性。DPPH法、ABTS法和FRAP法表明外源绿原酸提高了氧化胁迫下苹果叶片的抗氧化活性。此外，酚类物质的浓度显着增加响应绿原酸的应用后，甲基紫精暴露。此外，外源绿原酸还改变了抗氧化酶和酚类代谢相关基因的转录水平。这些结果表明，绿原酸可以有效的抗氧化剂保护植物免受氧化胁迫，通过增强抗氧化能力。
Considered to be an antioxidant, chlorogenic acid is involved in resistance to insects and pathogens, and beneficial effects on human health. However, little is known about the antioxidant functions of chlorogenic acid against oxidative stress in plants. In this study, we studied the potential role of exogenous chlorogenic acid in alleviating methyl viologen-mediated oxidative stress in detached leaves of apple (Malus domestica Borkh). The results showed that exogenous chlorogenic acid availably relieved the decreases in chlorophyll concentrations and maximum potential Photosystem II efficiency. Exogenous chlorogenic acid also effectively reduced membrane damage and lipid oxidation and stimulated activity of antioxidant enzymes, including peroxidase, catalase, and polyphenol oxidase. It is noteworthy that exogenous chlorogenic acid improves antioxidant activity of apple leaf under oxidative stress using DPPH, ABTS, and FRAP assays. In addition, the concentration of phenolics increased remarkably in response to chlorogenic acid application after methyl viologen exposure. Furthermore, exogenous chlorogenic acid also changed the transcription levels of related genes for antioxidant enzymes and phenolic metabolism. These results suggest that chlorogenic acid can be effective antioxidants protecting the plants against oxidative stress by enhancing antioxidant capacity.