CONTROL OF TRANSLATION BY MESSENGER-RNA SECONDARY STRUCTURE IN ESCHERICHIA-COLI - A QUANTITATIVE-ANALYSIS OF LITERATURE DATA
CONTROL OF TRANSLATION BY MESSENGER-RNA SECONDARY STRUCTURE IN ESCHERICHIA-COLI - A QUANTITATIVE-ANALYSIS OF LITERATURE DATA
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DOI：
10.1006/jmbi.1994.1714
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发表时间：
1994-11-25
影响因子：
5.6
通讯作者：
VANDUIN, J
中科院分区：
文献类型：
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作者：
DESMIT, MH;VANDUIN, J
大肠杆菌的翻译效率受mRNA在翻译起始区的二级结构的强烈控制。我们之前已经证明，RNA噬菌体MS2的衣蛋白基因产生的蛋白质与核糖体结合位点展开的mRNA分子的比例直接相关。这个分数是由局部二级结构的自由能(G（f)(0)）决定的。我们现在对其他四个核糖体结合位点的已发表数据进行了类似的分析。结果在定量上与MS2涂层蛋白基因的关系一致。翻译的效率取决于核糖体结合位点结构的整体稳定性，无论起始密码子本身是否碱基配对。弱于-6 kcal/mol的结构通常不会降低平移效率。在这个阈值以下，所有系统的表达量每减少-1.4 kcal/mol，就会减少10倍，正如理论预测的那样。
Translational efficiency in Escherichia coli is strongly controlled by the secondary structure of the mRNA in the translational initiation region. We have previously shown that protein production from the coat-protein gene of RNA bacteriophage MS2 is directly related to the fraction of mRNA molecules in which the ribosome binding site is unfolded. This fraction is dictated by the free energy (Delta G(f)(0)) of the local secondary structure. We now present a similar analysis of published data on four other ribosome binding sites. The results conform quantitatively to the same relationship as found for the MS2 coat-protein gene. The efficiency of translation is determined by the overall stability of the structure at the ribosome binding site, whether the initiation codon itself is base-paired or not. Structures weaker than -6 kcal/mol usually do not reduce translational efficiency. Below this threshold, all systems show a tenfold decrease in expression for every -1.4 kcal/mol, as predicted from theory.