Drosophila female sterile (1) homeotic is a multifunctional transcriptional regulator that is modulated by ras signaling
Drosophila female sterile (1) homeotic is a multifunctional transcriptional regulator that is modulated by ras signaling
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DOI：
10.1002/dvdy.21432
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Faller, Douglas V.
中科院分区：
文献类型：
--
作者：
Florence, Brian L.;Faller, Douglas V.
果蝇 (fs(1)h) 基因编码小 (Fs(1)hS) 和大 (Fs(1)hL) 染色质结合 BET 蛋白转录因子亚型。合子突变导致致死或雌性不育，而母本突变导致节段缺失和胸部同源异型转化。在这里，我们描述了新的 fs(1)h 胚胎表型：合子突变体中头部的同源异形和母体突变体中头部和尾部区域的缺失，类似于由显性躯干 (tor(D)) 等位基因引起的情况。 tor 通过经典 Ras 途径、通过 Groucho (Gro)、Capicua (Cic) 以及可能的 Grainy-head (Grh) 阻遏物的失活来激活 tailless (tll) 和 hiickebein (hkb) 的转录。 tailless 和 hiickebein 的表达在 fs(1)h 母体突变体中被去抑制，如在 tor(D)、gro、grh 和 cic 突变体动物中，表明 fs(1)h 对于 tll 和 hkb 抑制也是必要的。这些数据将 Ras 信号传导与染色质结合转录因子 Fs(1)h 的调节联系起来，表明 Ras 可以调节基因表达的新机制。
The Drosophila (fs(1)h) gene encodes small (Fs(1)hS) and large (Fs(1)hL) chromatin-binding BET protein transcription factor isoforms. Zygotic mutations cause either lethality or female sterility, whereas maternal mutations cause segmental deletions and thoracic homeotic transformations. Here, we describe novel fs(1)h embryonic phenotypes: homeosis of the head in zygotic mutants and deletion of head and tail regions in maternal mutants, similar to those caused by dominant torso (tor(D)) alleles. tor activates transcription of tailless (tll) and hiickebein (hkb) by means of a canonical Ras pathway, through inactivation of Groucho (Gro), Capicua (Cic) and, possibly, Grainy-head (Grh) repressors. Expression of both tailless and hiickebein are de-repressed in fs(1)h maternal mutants, as in tor(D), gro, grh, and cic mutant animals, indicating fs(1)h is also necessary for tll and hkb repression. These data link Ras signaling with modulation of a chromatin-binding transcription factor, Fs(1)h, suggesting a novel mechanism by which Ras can modulate gene expression.