Physiological response and morphological changes of Heterosigma akashiwo to an algicidal compound prodigiosin.
Physiological response and morphological changes of Heterosigma akashiwo to an algicidal compound prodigiosin.
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DOI：
10.1016/j.jhazmat.2019.121530
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发表时间：
2019-10
影响因子：
13.6
通讯作者：
Su Zhang;Wei Zheng;Hui Wang
中科院分区：
文献类型：
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作者：
Su Zhang;Wei Zheng;Hui Wang
有害藻华（HAB）在世界各地发生，对人类生活和海洋生态系统产生严重的负面影响。杀藻化合物灵菌红，由杀藻细菌 Hahelasp 分泌。 KA22可以溶解有害藻类赤潮异藻。本研究旨在探讨灵菌红素对幽门螺杆菌的杀藻机制。 akashiwo通过检测H的生理和形态反应。 akashiwoto 存在灵菌红。结果表明，灵菌红对H.赤潮的浓度为3μg/mL。在此浓度下，微藻的叶绿素和蛋白质水平显着下降，而丙二醛水平则上升。随着活性氧（ROS）的快速减少，抗坏血酸含量以及超氧化物歧化酶和过氧化物酶活性快速增加。对于3 μg/mL灵菌红素处理组，与光合作用和呼吸相关的基因转录在12小时时被显着抑制，而呼吸相关基因的转录在24小时时增加。总的来说，结果表明灵菌红可以通过诱导 ROS 过量产生来杀死微藻，这会破坏细胞完整性并改变抗氧化系统水平和功能基因表达。我们的结果表明，灵菌红是一种有效控制有害藻类的杀藻剂。
Harmful algal blooms (HABs) occur all over the world, producing severely negative effects on human life as well as on marine ecosystems. The algicidal compound, prodigiosin, secreted by algicidal bacteriaHahellasp. KA22 can lyse the harmful algaHeterosigma akashiwo. This study is aimed to investigate the algicidal mechanism of prodigiosin againstH. akashiwoby detecting physiological and morphological responses ofH. akashiwoto presence of prodigiosin. The results indicated that prodigiosin showed strong algicidal effects onH. akashiwoat the concentration of 3 μg/mL. Chlorophyllaand protein levels of the microalgae decreased significantly while malonaldehyde levels increased at this concentration. Contents of ascorbic acid and activities of superoxide dismutase and peroxidase increased fast with the quick decrease of the reactive oxygen species (ROS). For the 3 μg/mL prodigiosin treatment group, transcription of genes related to photosynthesis and respiration were significantly inhibited at 12 h while respiration related genes increased at 24 h. Collectively, the results indicated that prodigiosin could kill the microalgae by inducing ROS overproduction which could destroy the cell integrity and change the antioxidant system levels and functional gene expression. Our results demonstrated that prodigiosin is an effective algicide for the control of harmful algae.