Hyaluronan based porous nano-particles enriched with growth factors for the treatment of ulcers: a placebo-controlled study
Hyaluronan based porous nano-particles enriched with growth factors for the treatment of ulcers: a placebo-controlled study
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DOI：
10.1007/s10856-008-3566-3
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发表时间：
2009
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影响因子：
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通讯作者：
Barbara Zavan;Vincenzo Vindigni;K. Vezzù;G. Zorzato;C. Luni;Giovanni Abatangelo;N. Elvassore;R. Cortivo
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文献类型：
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作者：
Barbara Zavan;Vincenzo Vindigni;K. Vezzù;G. Zorzato;C. Luni;Giovanni Abatangelo;N. Elvassore;R. Cortivo
本研究描述了透明质酸为基础的多孔微粒的生产半连续气体抗溶剂（GAS）沉淀过程中使用的生长因子输送系统在体内治疗溃疡。调节操作工艺条件，例如压力、喷嘴直径和HYAFF 11 ®溶液浓度，以在形态和尺寸方面优化颗粒生产。扫描电子显微镜（SEM）和光散射表明，具有300和900 nm的尺寸的多孔纳米结构的颗粒具有高的比表面，适合于从聚合物基质内的水性环境中吸收生长因子。水作为增塑剂，促进生长因子的吸收。通过差示扫描量热法（DSC）分析HYAFF 11 ®基质内的水含量。利用荧光染料和生长因子开发了吸收过程。免疫组织化学分析证实了生长因子的高吸收效率，并生成数学模型来量化和鉴定聚合物基质内生长因子释放的体外动力学。进行了体内实验，目的是优化PDGF的定时和局部释放，以促进全层伤口的最佳组织修复和再生。
The present study describes the production of hyaluronan based porous microparticles by a semi-continuous gas anti-solvent (GAS) precipitation process to be used as a growth factor delivery system for in vivo treatment of ulcers. Operative process conditions, such as pressure, nozzle diameter and HYAFF11®solution concentrations, were adjusted to optimize particle production in terms of morphology and size. Scanning electron microscopy (SEM) and light scattering demonstrated that porous nano-structured particles with a size of 300 and 900 nm had a high specific surface suitable for absorption of growth factors from the aqueous environment within the polymeric matrix. Water acted as a plasticizer, enhancing growth factor absorption. Water contents within the HYAFF11®matrix were analyzed by differential scanning calorimetry (DSC). The absorption process was developed using fluorescence dyes and growth factors. Immunohistochemical analysis confirmed the high efficiency of absorption of growth factor and a mathematical model was generated to quantify and qualify the in vitro kinetics of growth factor release within the polymeric matrix. In vivo experiments were performed with the aim to optimize timed and focal release of PDGF to promote optimal tissue repair and regeneration of full-thickness wounds.