Multi-components of T2 relaxation in ex vivo cartilage and tendon
Multi-components of T2 relaxation in ex vivo cartilage and tendon
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DOI：
10.1016/j.jmr.2009.02.005
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Xia, Yang
中科院分区：
文献类型：
--
作者：
Zheng, ShaoKuan;Xia, Yang
在软骨和肌腱的T-2松弛的多个组件进行了研究显微镜MRI（μ MRI）在13和26 μ m的横向分辨率。两种成像方案用于量化标本中的T-2松弛，5点采样和60点采样。多指数和非负最小二乘（NNLS）拟合方法被用来分析μ MRI信号。当成像体素尺寸为6.76 x 10（-4）mm（3）并且在显微成像实验中的实际信噪比（SNR）的限制内时，我们发现（1）犬肌腱具有多个T-2成分;（2）牛鼻软骨具有单个T-2成分;以及（3）犬关节软骨具有单个T-2成分。发现5点和60点方法的T-2曲线在关节软骨中是一致的。此外，发现通过胰蛋白酶消化方法消耗软骨中的糖胺聚糖组分导致关节软骨中的T-2松弛增加9.81-20.52%，这取决于组织样本在磁场中取向的角度。(C)2009 Elsevier Inc. All rights reserved.
The multi-components of T-2 relaxation in cartilage and tendon were investigated by microscopic MRI (mu MRI) at 13 and 26 mu m transverse resolutions. Two imaging protocols were used to quantify T-2 relaxation in the specimens, a 5-point sampling and a 60-point sampling. Both multi-exponential and non-negative-least-square (NNLS) fitting methods were used to analyze the mu MRI signal. When the imaging voxel size was 6.76 x 10(-4) mm(3) and within the limit of practical signal-to-noise ratio (SNR) in microscopic imaging experiments, we found that (1) canine tendon has multiple T-2 components; (2) bovine nasal cartilage has a single T-2 component; and (3) canine articular cartilage has a single T-2 component. The T-2 profiles from both 5-point and 60-point methods were found to be consistent in articular cartilage. In addition, the depletion of the glycosaminoglycan component in cartilage by the trypsin digestion method was found to result in a 9.81-20.52% increase in T-2 relaxation in articular cartilage, depending upon the angle at which the tissue specimen was oriented in the magnetic field. (C) 2009 Elsevier Inc. All rights reserved.