Ultrastructural and cytochemical studies of the fate of unsecreted collagen precursors after administration of colchicine to mice.
Ultrastructural and cytochemical studies of the fate of unsecreted collagen precursors after administration of colchicine to mice.
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对小鼠给予秋水仙碱后未分泌的胶原前体的命运的超微结构和细胞化学研究。
DOI：
10.1159/000145968
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Garant,PR
中科院分区：
文献类型：
--
作者：
Cho,MI;Garant,PR
秋水仙碱的施用破坏了高尔基复合体的正常组织，并阻断了小鼠牙周膜成纤维细胞中胶原前体的分泌。随后通过电子显微镜观察高尔基体源性囊泡和新形成的致密体中未分泌的胶原前体的命运。观察到球囊内容物沿着逐渐冷凝，同时电子致密和电子透明材料发生相分离。融合的囊与致密的分泌体产生较大的内含物（斑马体; ZB）充满了电子致密和电子透明的材料的组合。在某些ZB中，这些材料似乎折叠成纤维状单元。纤维单位被乌洛托品银染色，就像正常的胶原纤维一样。这些结果表明，未分泌的胶原蛋白前体积累在囊泡隔室内，部分聚合可以发生。这一发现可以解释一些报告的细胞内胶原纤维的病理改变结缔组织的成纤维细胞。
The administration of colchicine disrupts the normal organization of the Golgi complex and blocks the secretion of collagen precursors in periodontal ligament fibroblasts of the mouse. The fate of the unsecreted collagen precursors contained in Golgi-derived saccules and newly formed dense bodies was followed by electron microscopy. A progressive condensation of saccule content along with phase separation of electron-dense and electron-lucent material was observed. Fusion of saccules with dense secretory bodies gave rise to larger inclusions (zebra bodies; ZB) filled with a combination of electron-dense and electron-lucent material. In some ZB, these materials appeared to polymerize into fibrillar units. The fibrillar units stained with silver methenamine like normal collagenous fibrils. These results suggest that unsecreted collagen precursors accumulate in vesicular compartments within which partial polymerization can occur. This finding may explain some reports of intracellular collagenous fibrils in fibroblasts of pathologically altered connective tissues.