BIOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A SUBUNIT OF A CALMODULIN-DEPENDENT PROTEIN-KINASE
BIOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A SUBUNIT OF A CALMODULIN-DEPENDENT PROTEIN-KINASE
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DOI：
10.1073/pnas.80.23.7357
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
ERONDU, NE
中科院分区：
文献类型：
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作者：
KENNEDY, MB;BENNETT, MK;ERONDU, NE
通过2个生物化学标准和1个免疫化学标准，主要突触后密度蛋白（mPSDp）与50千道尔顿（kDa）的α-突触后密度蛋白是不可区分的。脑钙调素依赖性蛋白激酶亚基。在NaDodSO_4/聚丙烯酰胺凝胶上，两种蛋白质共迁移。2种蛋白的碘化胰蛋白酶肽图谱相同。[从鼠杂交瘤细胞]产生的抗蛋白激酶的单克隆抗体（6 G9）在免疫印迹上与粗[大鼠]脑匀浆中的单个50 kDa条带结合，并与α-半乳糖苷酶和α-半乳糖苷酶结合。纯化的激酶的亚基和来自突触后密度级分的mPSDp。纯化的激酶全酶还含有称为β的60-kDa亚基。β的肽图谱的比较。与来自突触后密度部分的60-kDa蛋白质的图谱表明β存在于那里，但不是在该MW范围内存在的唯一蛋白质。钙调素依赖性蛋白激酶是突触后密度组分的主要成分，因此可能是I型突触后密度的组成部分。
By 2 biochemical and 1 immunochemical criteria the major postsynaptic density protein (mPSDp) is indistinguishable from the 50-kilodalton (kDa) .alpha. subunit of a brain calmodulin-dependent protein kinase. The 2 proteins comigrate on NaDodSO4/polyacrylamide gels. Iodinated tryptic peptide maps of the 2 are identical. A monoclonal antibody (6G9) that was raised [from murine hybridoma cells] against the protein kinase binds on immunoblots to a single 50 kDa band in crude [rat] brain homogenates and to both the .alpha. subunit of the purified kinase and the mPSDp from postsynaptic density fractions. The purified kinase holoenzyme also contains a 60-kDa subunit termed .beta.. A comparison of the peptide map of .beta. with the maps of 60-kDa proteins from the postsynaptic density fraction suggests that .beta. is present there but is not the only protein present in this MW range. The calmodulin-dependent protein kinase is a major constituent of the postsynaptic density fraction and thus may be a component of type I postsynaptic densities.