mRNA vaccination induces tick resistance and prevents transmission of the Lyme disease agent
mRNA vaccination induces tick resistance and prevents transmission of the Lyme disease agent
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DOI：
10.1126/scitranslmed.abj9827
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发表时间：
2021-11-17
影响因子：
17.1
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
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作者：
Sajid, Andaleeb;Matias, Jaqueline;Fikrig, Erol
肩突硬蜱传播许多引起人类疾病的病原体，包括伯氏疏螺旋体。获得性耐药。由于反复接触蜱虫而引起的肩胛肌的炎症具有预防蜱虫传播的传染病的潜力，并且已经假定唾液蛋白有助于该过程。我们检测了含有核苷修饰的脂质纳米颗粒编码19 I的mRNA的能力。肩胛肌唾液蛋白（19ISP），以增强识别蜱叮咬和减少I。肩胛肌充血，从而防止B。伯氏感染用19ISP mRNA疫苗免疫豚鼠，随后用I.肩胛肌在蜱虫开始附着后不久，给予19ISP的动物在叮咬部位出现红斑，这些蜱虫进食不良，表现为早期脱离和饱食体重下降。19 ISP免疫也阻碍了B。豚鼠中的伯氏菌传播。I后早期有效诱导局部发红。肩胛肌附着和蜱不能摄取正常血餐表明，19ISP可以单独使用或与传统的基于病原体的疫苗联合使用，用于预防莱姆病和潜在的其他蜱传播的感染。
Ixodes scapularis ticks transmit many pathogens that cause human disease, including Borrelia burgdorferi. Acquired resistance to I. scapularis due to repeated tick exposure has the potential to prevent tick-borne infectious diseases, and salivary proteins have been postulated to contribute to this process. We examined the ability of lipid nanoparticle-containing nucleoside-modified mRNAs encoding 19 I. scapularis salivary proteins (19ISP) to enhance the recognition of a tick bite and diminish I. scapularis engorgement on a host and thereby prevent B. burgdorferi infection. Guinea pigs were immunized with a 19ISP mRNA vaccine and subsequently challenged with I. scapularis. Animals administered 19ISP developed erythema at the bite site shortly after ticks began to attach, and these ticks fed poorly, marked by early detachment and decreased engorgement weights. 19ISP immunization also impeded B. burgdorferi transmission in the guinea pigs. The effective induction of local redness early after I. scapularis attachment and the inability of the ticks to take a normal blood meal suggest that 19ISP may be used either alone or in conjunction with traditional pathogen-based vaccines for the prevention of Lyme disease, and potentially other tick-borne infections.