Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
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DOI：
10.1016/j.ecoenv.2021.112907
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发表时间：
2021-10-19
影响因子：
6.8
通讯作者：
Hou, Hongwei
中科院分区：
文献类型：
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作者：
Chen, Yan;Li, Gaojie;Hou, Hongwei
镉作为一种污染物，对环境造成严重影响，并对生物体造成危害。它可以通过植物中的天然抗性相关巨噬细胞蛋白（Nramp）从环境中摄取。然而，在多根螺旋藻中Nramp转运蛋白基因的离子吸收功能还未见报道。本研究对S.多根的克隆和功能分析。甘草和酵母。在镉胁迫下测定了野生型和转基因株系的生长参数和理化指标。结果表明，SpNramp1、SpNramp2和SpNramp3均为质膜定位转运蛋白，并在酵母中证实了它们在转运Cd中的作用。In S.在多根中，SpNramp1过表达显著提高了Cd、Fe、Mn含量和鲜重。SpNramp2过表达增加Mn和Cd。SpNramp3过表达增加Fe和Mn浓度。这些结果表明，SpNramp1、SpNramp2和SpNramp3对离子吸收具有不同的偏好。两个S获得了多根转基因株系（OE1和OE3）。其中一株（OE1）表现出较强的积累能力，另一株（OE3）表现出较强的耐性。这项研究提供了新的见解SpNramp1，SpNramp2，和SpNramp3的功能，并获得重要的富集线（OE1）操纵镉积累，植物修复，生态安全。
As a pollutant, Cd causes severe impact to the environment and damages living organisms. It can be uptaken from the environment by the natural resistance-associated macrophage protein (Nramp) in plants. However, the ion absorption function of Nramp transporter genes in Spirodela polyrhiza has not been reported. In this study, SpNramp1, SpNramp2, and SpNramp3 from S. polyrhiza were cloned and their functions were analyzed in S. polyrhiza and yeast. Growth parameters and physicochemical indices of wild-type and transgenic lines were measured under Cd stress. Results revealed that SpNramp1, SpNramp2, and SpNramp3 were identified as plasma membrane-localized transporters, and their roles in transporting Cd were verified in yeast. In S. polyrhiza, SpNramp1 overexpression significantly increased the content of Cd, Fe, Mn, and fresh weight. SpNramp2 overexpression increased Mn and Cd. SpNramp3 overexpression increased Fe and Mn concentrations. These results indicate that SpNramp1, SpNramp2, and SpNramp3 had a different preference for ion absorption. Two S. polyrhiza transgenic lines (OE1 and OE3) were obtained. One of them (OE1) showed a stronger accumulation ability, and the other one (OE3) exhibited tolerance capacity to Cd. This study provides new insight into the functions of SpNramp1, SpNramp2, and SpNramp3 and obtains important enrichment lines (OE1) for manipulating Cd accumulation, phytoremediation, and ecological safety.