lncRNA-AC130710 targeting by miR-129-5p is upregulated in gastric cancer and associates with poor prognosis
lncRNA-AC130710 targeting by miR-129-5p is upregulated in gastric cancer and associates with poor prognosis
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miR-129-5p 靶向的 lncRNA-AC130710 在胃癌中上调并与不良预后相关
DOI：
10.1007/s13277-014-2274-5
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发表时间：
2014-10-01
期刊：
影响因子：
--
通讯作者：
Guo, Junming
中科院分区：
文献类型：
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作者：
Xu, Chunjing;Shao, Yongfu;Guo, Junming
长链非编码RNA（longnoncodingRNAs，lncRNAs）在肿瘤的发生、发展中起着重要作用。然而，lncRNA在胃癌诊断和治疗中的意义在很大程度上是未知的。本研究以其中一种lncRNA AC 130710为研究对象。收集78例胃癌患者的癌组织及癌旁组织。通过逆转录聚合酶链反应（RT-PCR）检测AC 130710水平。进一步分析AC 130710水平与胃癌患者临床病理因素的关系。最后，对AC 130710在胃癌细胞中高表达的分子机制进行了初步探讨。结果表明，胃癌组织中AC 130710的表达明显高于癌旁组织（P< 0.05）。其表达水平与肿瘤大小（P= 0.013）、TNM分期（P= 0.030）和远处转移（P= 0.018）显著相关。MGC-803细胞中AC 130710的表达明显高于正常胃粘膜细胞系GES-1（P< 0.001）。此外，miR-129- 5 p可能在胃癌细胞AC 130710的下调中发挥重要作用。提示lncRNA-AC 130710可能是胃癌预后的潜在肿瘤标志物。
Long noncoding RNAs (lncRNAs) play an important role in cancer occurrence and development. However, there is largely unknown about lncRNAs significance in the diagnosis and treatment of gastric cancer. In our study, we focused on AC130710, one of lncRNAs. Gastric cancer tissues and adjacent tissues were gathered from 78 patients with gastric cancer. The AC130710 levels were detected by reverse transcription polymerase chain reaction (RT-PCR). Then, we further analyzed the association between AC130710 level and the clinicopathological factors of patients with gastric cancer. Finally, the molecular mechanism underling AC130710 highly expressed in gastric cancer cells was explored. The results showed that AC130710 in cancer tissues from patients with gastric cancer was significantly higher than those in adjacent noncancerous tissues (P< 0.05). Its expression level was significantly associated with tumor size (P= 0.013), tumor-node-metastasis (TNM) stages (P= 0.030), and distal metastasis (P= 0.018). AC130710 expression in MGC-803 was significantly higher than that in normal gastric mucosa cell line GES-1 (P< 0.001). Moreover, miR-129-5p may play an important role in the downregulation of AC130710 in gastric cancer cells. These results indicated that lncRNA-AC130710 may be a potential tumor marker for gastric cancer prognosis.