DISTINCT ASPECTS OF NEURONAL DIFFERENTIATION ENCODED BY FREQUENCY OF SPONTANEOUS CA2+ TRANSIENTS
DISTINCT ASPECTS OF NEURONAL DIFFERENTIATION ENCODED BY FREQUENCY OF SPONTANEOUS CA2+ TRANSIENTS
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DOI：
10.1038/375784a0
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发表时间：
1995-06-29
期刊：
影响因子：
64.8
通讯作者：
SPITZER, NC
中科院分区：
文献类型：
--
作者：
GU, XN;SPITZER, NC
细胞内钙（Ca-i(2+)）的瞬时增加刺激可激活蛋白激酶（1-3），调节转录（4-9）并影响运动和形态，发育中的神经元产生自发的Ca-i（2+）瞬态，但它们在指导神经元分化中的作用及其编码信息的方式尚不清楚。我们在整个早期发育过程中对脊髓神经元中的Ca2+进行了成像，并发现两种类型的自发事件，尖峰和波，以不同的频率表达。神经元分化是改变时，他们被消除，防止Ca2+内流。重新施加不同频率的Ca2+升高模式表明，自然尖峰活动足以促进正常的神经递质表达和通道成熟，而波活动足以调节神经突延伸。细胞内装载的BAPTA对自发Ca2+升高的抑制表明它们也是分化所必需的。Ca2+瞬态似乎在其频率上编码信息，就像动作电位一样，尽管它们的持续时间长10(4)倍，频率较低，并且实现了内在的发展程序。
STIMULATION of transient increases in intracellular calcium (Ca-i(2+)) activates protein kinases(1-3), regulates transcription(4-9) and influences motility and morphology Developing neurons generate spontaneous Ca-i(2+) transients, but their role in directing neuronal differentiation and the way in which they encode information are unknown. Acre we image Ca2+ in spinal neurons throughout an extended period of early development, and find that two types of spontaneous events, spikes and waves, are expressed at distinct frequencies. Neuronal differentiation is altered when they are eliminated by preventing Ca2+ influx. Reimposing different frequency patterns of Ca2+ elevation demonstrates that natural spike activity is sufficient to promote normal neurotransmitter expression and channel maturation, whereas wave activity is sufficient to regulate neurite extension. Suppression of spontaneous Ca2+ elevations by BAPTA loaded intracellularly indicates that they are also necessary for differentiation. Ca2+ transients appear to encode information in their frequency, like action potentials, although they are 10(4) times longer in duration and less frequent, and implement an intrinsic development programme.