A Unified Approach to Analysing Cell Motility
A Unified Approach to Analysing Cell Motility
复制标题
分析细胞运动性的统一方法
作者：
G. Dunn;A. F. Brown
摘要 培养物中细胞运动的定量分析具有几个重要的功能。首先，它给出了运动过程的简洁而准确的描述，并且可以检测由于不同的基因组成或实验条件而导致的运动的细微差异。其次，它的客观性意味着结果可以精确地传达并明确地用于检验有关运动性的假设。第三，它可以用来推导与运动过程具有相同统计特性的数学模型，从而阐明运动机制。在本文中，我们介绍了在各种情况下分析细胞运动的一般程序。我们描述了一个试点项目，用于分析从随机移动的成纤维细胞获得的简单几何数据。最后，作为一个例子，我们展示了对成纤维细胞易位的分析如何能够深入了解运动机制，而这可能是任何其他方法都无法获得的。
SUMMARY The quantitative analysis of cell motility in culture has several important functions. First, it gives a concise and accurate description of the motile process and can detect subtle differences in motility due to different genetic makeup or experimental conditions. Second, its objectivity means that results can be communicated precisely and used unambiguously to test hypotheses about motility. Third, it may be used to derive a mathematical model with the same statistical properties as the motile process and thus elucidate the mechanism of motility. In this paper, we introduce a general procedure for analysing cell motility in a wide variety of circumstances. We describe a pilot project for the analysis of simple geometrical data obtained from randomly moving fibroblasts. Finally, as an example, we show how an analysis of the translocation of the fibroblasts can lead to insights into the mechanism of motility that are arguably not obtainable by any other approach.