Distribution of multiple peptidoglycan recognition proteins in the tissues of Pacific oyster, Crassostrea gigas
Distribution of multiple peptidoglycan recognition proteins in the tissues of Pacific oyster, Crassostrea gigas
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DOI：
10.1016/j.cbpb.2008.04.011
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发表时间：
2008-08-01
影响因子：
2.2
通讯作者：
Takahashi, Keisuke G.
中科院分区：
文献类型：
--
作者：
Itoh, Naoki;Takahashi, Keisuke G.
肽聚糖识别蛋白（PGRPs）是一种特异性结合肽聚糖的模式识别受体，是细菌细胞壁的主要成分。一般来说，pgrp负责识别无脊椎动物的细菌入侵。PGRP全长cdna分别为CgPGRP-S1S、-S1L、-S2和-S3。同源性和结构域搜索将这些CgPGRPs归类为细胞外PGN识别的短型PGRPs。在所有cgpgrp中均预测到酰胺酶活性，并且在CgPGRP-S1S和-S1L中发现了防御素样结构域，这表明它们也可能具有抗菌蛋白的功能。系统发育分析表明，CgPGRPs与其他基因关系密切，但在组织中表达模式不同；CgPGRP-S1S在膜和鳃中，-S1L在膜中，-S2在血细胞中，-S3在消化憩室中。CgPGRPs似乎在其相应的表达组织中调查细菌入侵。这是首次报道双壳类软体动物可能具有分布在不同组织中的多种pgrp识别系统。(c) 2008爱思唯尔公司版权所有。
Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that specifically bind to peptidoglycans, a major component of bacterial cell wall. Generally, PGRPs are responsible for recognition of bacterial invasion in invertebrates. Full length cDNAs of PGRP, designated as CgPGRP-S1S, -S1L, -S2 and -S3, were identified from the Pacific oyster, Crassostrea gigas. Homology and domain searches classified these CgPGRPs as short-type PGRPs for extracellular PGN recognition. Amidase activity was predicted in all CgPGRPs, and defensin-like domains were found in CgPGRP-S1S and -S1L, suggesting that they may also function as antimicrobial proteins. Although phylogenetic analysis indicated that CgPGRPs are closely related to each other, they showed different tissue expression patterns; CgPGRP-S1S in the mantle and the gill, -S1L in the mantle, -S2 in the hemocytes and -S3 in the digestive diverticula. The CgPGRPs seem to survey bacterial invasion in their corresponding expression tissues. This is the first report of the possibility that bivalve mollusks have PGN recognition systems as suggested by the identification of multiple PGRPs distributed in various tissues. (c) 2008 Elsevier Inc. All rights reserved.