Dual aggregation-induced emission for enhanced fluorescence sensing of furin activity in vitro and in living cells
Dual aggregation-induced emission for enhanced fluorescence sensing of furin activity in vitro and in living cells
复制标题
双聚集诱导发射，增强体外和活细胞中弗林蛋白酶活性的荧光传感
DOI：
10.1039/c6cc09106g
复制
发表时间：
2017-01-18
影响因子：
4.9
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
--
作者：
Liu, Xiaomei;Liang, Gaolin
聚集诱导发射(AIE)效应近年来被广泛应用于生物标志物传感。在这项工作中，通过将生物相容的缩合反应与AIE荧光素相结合，我们合理地设计了一种“SMART‘双AIE探针Ac-ArgVal-Arg-Arg-Cys(StBu)-Lys(TPE)-CBT(1)，用于增强体外和活细胞中对Furin的荧光传感活性。与单一AIE探针Ac-Arg-Val-Arg-Arg-Lys(TPE)-OH(1-Ctrl)相比，1在体外和活细胞中的荧光发射分别增加了1.7倍和3.4倍。我们预计，在不久的将来，我们的酶指示双AIE的“智能”策略可以广泛应用于检测(或成像)体外甚至体内的酶活性，并显著提高灵敏度。
The aggregation-induced emission (AIE) effect has recently been widely applied for biomarker sensing. But developing "smart'' strategies to effectively aggregate the AIE fluorogen and additionally enhance the fluorescence emission remain challenging. In this work, by integrating a biocompatible condensation reaction with an AIE fluorogen, we rationally designed a "smart'' dual AIE probe Ac-ArgVal- Arg-Arg-Cys(StBu)-Lys(TPE)-CBT (1) for enhanced fluorescence sensing furin activity in vitro and in living cells. Compared with the single AIE probe Ac-Arg-Val-Arg-Arg-Lys(TPE)-OH (1-Ctrl) which also subjects to furin cleavage, fluorescence emissions of 1 were additionally enhanced 1.7 fold and 3.4 fold in vitro and in living cells, respectively. We envision that, in the near future, our "smart'' strategy of enzyme-instructed dual AIE could be widely applied for sensing (or imaging) enzyme activity in vitro and even in vivo with dramatically enhanced sensitivity.