Rhizopine biosensors for plant-dependent control of bacterial gene expression.
Rhizopine biosensors for plant-dependent control of bacterial gene expression.
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DOI：
10.1111/1462-2920.16288
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发表时间：
2023-02
影响因子：
5.1
通讯作者：
Poole, Philip S.
中科院分区：
文献类型：
--
作者：
Haskett, Timothy L.;Geddes, Barney A.;Paramasivan, Ponraj;Green, Patrick;Chitnavis, Samir;Mendes, Marta D.;Jorrin, Beatriz;Knights, Hayley E.;Bastholm, Tahlia R.;Ramsay, Joshua P.;Oldroyd, Giles E. D.;Poole, Philip S.
可以利用植物和微生物之间的工程信号来建立植物生长促进细菌和环境中目标作物之间的宿主特异性。我们之前设计了根碱信号通路，促进了根碱生产（RhiP）植物和模型菌株之间的排他性信号传导。在这里，我们深入分析了细菌中根碱诱导的表达。我们表征了两个根碱诱导型启动子，并探索了根碱生物传感器质粒的细菌宿主范围。通过调节根碱摄取基因的表达，我们还构建了一种新的生物传感器质粒pSIR05，其对体外宿主细胞生长的影响最小，并且与先前描述的生物传感器质粒pSIR02相比，在RhiP大麦根上原位表达的稳定性显著提高。我们证明了携带pSIR05的Azorhizobium caulinodans细胞的亚群可以在定殖RhiP大麦根时感测根碱并激活基因表达。然而，与野生型亲本菌株相比，这些细菌对于RhiP大麦根的定殖有轻度缺陷。这项工作为建立更强大的植物依赖性细菌基因表达控制提供了进展，并强调了实现这一目标的关键挑战。
Engineering signalling between plants and microbes could be exploited to establish host‐specificity between plant‐growth‐promoting bacteria and target crops in the environment. We previously engineered rhizopine‐signalling circuitry facilitating exclusive signalling between rhizopine‐producing (RhiP) plants and model bacterial strains. Here, we conduct an in‐depth analysis of rhizopine‐inducible expression in bacteria. We characterize two rhizopine‐inducible promoters and explore the bacterial host‐range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub‐population of Azorhizobium caulinodans cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing RhiP barley roots. However, these bacteria were mildly defective for colonization of RhiP barley roots compared to the wild‐type parent strain. This work provides advancement towards establishing more robust plant‐dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal.
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影响因子：
4.5
作者：
通讯作者：
--
影响因子：
5.2
作者：
Haskett TL;Knights HE;Jorrin B;Mendes MD;Poole PS
通讯作者：
Poole PS
DOI：
10.1073/pnas.2117465119
发表时间：
2022-04-19
影响因子：
11.1
作者：
通讯作者：
--
影响因子：
4.7
作者：
Boleta, Eduardo Henrique Marcandalli;Shintate Galindo, Fernando;Teixeira Filho, Marcelo Carvalho Minhoto
通讯作者：
Teixeira Filho, Marcelo Carvalho Minhoto
影响因子：
3.6
作者：
Belitsky, BR;Sonenshein, AL
通讯作者：
Sonenshein, AL