Lipidomics Analyses Reveal Temporal and Spatial Lipid Organization and Uncover Daily Oscillations in Intracellular Organelles
Lipidomics Analyses Reveal Temporal and Spatial Lipid Organization and Uncover Daily Oscillations in Intracellular Organelles
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DOI：
10.1016/j.molcel.2016.04.002
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发表时间：
2016-05-19
期刊：
影响因子：
16
通讯作者：
Asher, Gad
中科院分区：
文献类型：
--
作者：
Aviram, Rona;Manella, Gal;Asher, Gad
细胞已经进化出处理不相容过程的机制，通过生物钟的时间组织和脂质双层定义的细胞器内的空间区室化。脂质组学的最新进展导致了丰富的脂质种类的鉴定，但我们的知识，他们的时空组织是落后的。在这项研究中，我们定量表征了小鼠肝脏中的细胞核和线粒体脂质体，在不同的喂养方案，并在时钟中断的小鼠。我们的分析揭示了脂质类内和脂质类之间的潜在联系。值得注意的是，我们发现了细胞核和线粒体中脂质积累的昼夜振荡。这些振荡表现出相反的相位，并很容易响应喂养时间。此外，我们发现，昼夜节律钟协调细胞器之间的相位关系。总之，我们的研究提供了脂质组织的时间和空间描述，并揭示了细胞内细胞器昼夜节律的存在和协调。
Cells have evolved mechanisms to handle incompatible processes through temporal organization by circadian clocks and by spatial compartmentalization within organelles defined by lipid bilayers. Recent advances in lipidomics have led to identification of plentiful lipid species, yet our knowledge regarding their spatiotemporal organization is lagging behind. In this study, we quantitatively characterized the nuclear and mitochondrial lipidome in mouse liver throughout the day, upon different feeding regimens, and in clock-disrupted mice. Our analyses revealed potential connections between lipid species within and between lipid classes. Remarkably, we uncovered diurnal oscillations in lipid accumulation in the nucleus and mitochondria. These oscillations exhibited opposite phases and readily responded to feeding time. Furthermore, we found that the circadian clock coordinates the phase relation between the organelles. In summary, our study provides temporal and spatial depiction of lipid organization and reveals the presence and coordination of diurnal rhythmicity in intracellular organelles.