ART: a next-generation sequencing read simulator
ART: a next-generation sequencing read simulator
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DOI：
10.1093/bioinformatics/btr708
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发表时间：
2012-02-15
期刊：
影响因子：
5.8
通讯作者：
Marth, Gabor T.
中科院分区：
文献类型：
--
作者：
Huang, Weichun;Li, Leping;Marth, Gabor T.
ART是一组模拟工具，可生成合成的下一代测序读数。该功能对于用于下一代测序数据分析的测试和基准测试工具至关重要，包括读段比对、从头组装和遗传变异发现。ART通过使用内置的、技术特定的读取错误模型和在大型测序数据集中凭经验参数化的碱基质量值概况来模拟测序过程，从而生成模拟的测序读取。我们目前支持所有三个主要的商业下一代测序平台：罗氏的454，Illumina的Solexa和应用生物系统的SOLiD。ART还允许灵活地使用定制的读取错误模型参数和质量配置文件。
ART is a set of simulation tools that generate synthetic next-generation sequencing reads. This functionality is essential for testing and benchmarking tools for next- generation sequencing data analysis including read alignment, de novo assembly and genetic variation discovery. ART generates simulated sequencing reads by emulating the sequencing process with built-in, technology-specific read error models and base quality value profiles parameterized empirically in large sequencing datasets. We currently support all three major commercial next- generation sequencing platforms: Roche's 454, Illumina's Solexa and Applied Biosystems' SOLiD. ART also allows the flexibility to use customized read error model parameters and quality profiles.