Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants.
Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants.
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人类胎盘外植体中对 COVID-19 mRNA 疫苗暴露的最小 mRNA 摄取和炎症反应。
DOI：
10.1101/2023.02.01.23285349
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Gaw,StephanieL
中科院分区：
文献类型：
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作者：
Gonzalez,Veronica;Li,Lin;Buarpung,Sirirak;Prahl,Mary;Robinson,JoshuaF;Gaw,StephanieL
尽管普遍建议在怀孕期间接种 COVID-19 mRNA 疫苗，但接种率仍低于预期。关于 COVID-19 mRNA 疫苗暴露对人胎盘组织的直接影响的研究有限。使用原代人胎盘外植体模型，我们研究了两种常见 mRNA 疫苗（BNT162b2 Pfizer-BioNTech 或 mRNA-1273 Moderna）的摄取，以及暴露是否改变了绒毛细胞因子反应。将源自妊娠中期或晚期绒毛膜绒毛的外植体与超生理浓度的疫苗一起孵育，并在两个时间点进行分析。我们通过原位杂交和定量 RT-PCR 观察到胎盘外植体中 mRNA 疫苗的摄取极少。在多重免疫测定中，两种 mRNA 疫苗均未引发特异性或整体细胞因子反应。我们的结果表明，人类胎盘不容易吸收 COVID-19 mRNA 疫苗，也不会在接触后产生明显的炎症反应。
Despite universal recommendations for COVID-19 mRNA vaccination in pregnancy, uptake has been lower than desired. There have been limited studies of the direct impact of COVID-19 mRNA vaccine exposure in human placental tissue. Using a primary human placental explants model, we investigated the uptake of two common mRNA vaccines (BNT162b2 Pfizer-BioNTech or mRNA-1273 Moderna), and whether exposure altered villous cytokine responses. Explants derived from second or third trimester chorionic villi were incubated with vaccines at supraphysiologic concentrations and analyzed at two time points. We observed minimal uptake of mRNA vaccines in placental explants byin situhybridization and quantitative RT-PCR. No specific or global cytokine response was elicited by either of the mRNA vaccines in multiplexed immunoassays. Our results suggest that the human placenta does not readily absorb the COVID-19 mRNA vaccines nor generate a significant inflammatory response after exposure.