Effects of environmental chemicals on the proliferation and differentiation of neural stem cells
Effects of environmental chemicals on the proliferation and differentiation of neural stem cells
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环境化学物质对神经干细胞增殖和分化的影响
DOI：
10.1002/tox.22829
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发表时间：
2019-08-09
影响因子：
4.5
通讯作者：
Yang, Hui-fang
中科院分区：
文献类型：
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作者：
Huang, Min;Li, Yingying;Yang, Hui-fang
本研究旨在探讨甲基汞、百草枯和双酚A等环境化学物质对神经干细胞（NSCs）增殖和凋亡以及神经元分化相关基因表达水平的影响。本研究以来源于人脐带血的神经干细胞（HUCB-NSC）为细胞模型。采用CCK-8和流式细胞术检测甲基汞、百草枯和双酚A对HUCB-NSCs增殖和分化过程中细胞增殖和凋亡的影响。采用逆转录-聚合酶链反应和蛋白质印迹法检测神经元分化相关基因的表达。结果表明，甲基汞、百草枯和双酚A处理后，HUCB-NSCs的增殖受到明显抑制，细胞凋亡明显增加，Oct 4、Gdf 3和Sox 1的mRNA和蛋白表达水平下降，而Pax 6和Ngn 1的mRNA和蛋白表达水平升高。总之，本研究表明，环境化学物质可以损害神经干细胞的增殖和分化，这可能会导致神经系统的异常发育。
The objective of the present study was to explore the effects of environmental chemicals, such as methyl mercury, paraquat, and bisphenol A, on cell proliferation and apoptosis, as well as the expression levels of neuronal differentiation-related genes in neural stem cells (NSCs). NSCs originated from human umbilical cord blood (HUCB-NSCs) were used as cell models in the current study. CCK-8 and flow cytometry experiments were performed to assess the effects of methyl mercury, paraquat, and bisphenol A on the proliferation and apoptosis of HUCB-NSCs at different processes, including proliferation and differentiation stages. The expressions of neuronal differentiation-related genes were determined by reverse transcription-polymerase chain reaction and western blot analysis. The results showed that methyl mercury, paraquat, and bisphenol A treatments significantly inhibited cell proliferation and induced cell apoptosis in HUCB-NSCs, as well as decreased the expressions of Oct4, Gdf3, and Sox1, whereas increased Pax6 and Ngn1 expressions at both mRNA and protein levels. In conclusion, this study demonstrates that environmental chemicals can impair the proliferation and differentiation of NSCs, which may cause abnormal development of the nervous system.