Plasma membrane tension regulates eisosome structure and function
Plasma membrane tension regulates eisosome structure and function
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DOI：
10.1091/mbc.e19-04-0218
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发表时间：
2020-02-15
影响因子：
3.3
通讯作者：
Babst, Markus
中科院分区：
文献类型：
--
作者：
Appadurai, Daniel;Gay, Lincoln;Babst, Markus
酵母小体是酵母细胞表面的膜沟，已被证明在两个看似不同的途径中起作用，膜应激反应和营养转运蛋白的调节。我们发现，许多压力条件影响这两个途径，通过改变质膜张力，从而改变的形态和组成的eisosomes。例如，碱性应激引起细胞肿胀和内吞反应，它们一起增加膜张力，从而使eisosome变平。扁平化的eisosomes影响膜应激途径并释放营养转运蛋白，这有助于它们的下调。相反，葡萄糖饥饿或高渗休克引起细胞收缩，这导致膜松弛和Eisosomes加深。加深的Eisosomes能够捕获营养转运蛋白并保护它们免受快速内吞作用。因此，eisosomes似乎协调调节膜张力和营养转运蛋白的稳定性。
Eisosomes are membrane furrows at the cell surface of yeast that have been shown to function in two seemingly distinct pathways, membrane stress response and regulation of nutrient transporters. We found that many stress conditions affect both of these pathways by changing plasma membrane tension and thus the morphology and composition of eisosomes. For example, alkaline stress causes swelling of the cell and an endocytic response, which together increase membrane tension, thereby flattening the eisosomes. The flattened eisosomes affect membrane stress pathways and release nutrient transporters, which aids in their down-regulation. In contrast, glucose starvation or hyperosmotic shock causes cell shrinking, which results in membrane slack and the deepening of eisosomes. Deepened eisosomes are able to trap nutrient transporters and protect them from rapid endocytosis. Therefore, eisosomes seem to coordinate the regulation of both membrane tension and nutrient transporter stability.