Expression of the fibroblast growth factor-5 gene in the mouse embryo.
Expression of the fibroblast growth factor-5 gene in the mouse embryo.
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作者：
O. Haub;M. Goldfarb
成纤维细胞生长因子（FGF）是结构上相关的有丝分裂原，其可以调节多种细胞的分化。作为一个步骤，阐明这些因素之一所发挥的发展作用，我们已经使用原位杂交方法来研究小鼠F GF-5基因在胚胎发生过程中的表达。在发育中的小鼠胚胎中的七个不同位点检测到F gf-5 RNA：（1）着床后上胚层（2）内脏侧中胚层（3）侧体中胚层（E10 1/2- 1/2 1/2），（4）肌切开器（5）咀嚼肌（E11 1/2 - 14 1/2），（6）肢体间充质（E1/2 1/2 - 14 1/2），和（7）听神经节（E1/2 1/2-14 1/2）。在这些位点中的几个，表达在组织内空间受限。我们提供了几个关于FGF-5在小鼠发育中的作用的假设。
Fibroblast growth factors (FGFs) are structurally related mitogens that can regulate the differentiation of a wide variety of cells. As a step towards elucidating the developmental roles played by one of these factors, we have used in situ hybridization methods to examine expression of the murine F gf-5 gene during embryogenesis. F gf-5 RNA was detected at seven distinct sites in the developing mouse embryo: (1) postimplantation epiblast (embryonic day 5 1/4-7 1/2), (2) lateral splanchnic mesoderm (E9 1/2-10 1/2), (3) lateral somatic mesoderm (E10 1/2-12 1/2), (4) myotomes (E10 1/2-12 1/2), (5) mastication muscle (E11 1/2-14 1/2), (6) limb mesenchyme (E12 1/2-14 1/2), and (7) acoustic ganglion (E12 1/2-14 1/2). At several of these sites, expression is spatially restricted within the tissues. We offer several hypotheses regarding the roles of FGF-5 in murine development.