THE RAT-BRAIN SYNUCLEINS - FAMILY OF PROTEINS TRANSIENTLY ASSOCIATED WITH NEURONAL MEMBRANE
THE RAT-BRAIN SYNUCLEINS - FAMILY OF PROTEINS TRANSIENTLY ASSOCIATED WITH NEURONAL MEMBRANE
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DOI：
10.1016/0169-328x(91)90043-w
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发表时间：
1991-10-01
期刊：
影响因子：
--
通讯作者：
SCHELLER, RH
中科院分区：
文献类型：
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作者：
MAROTEAUX, L;SCHELLER, RH
突触核蛋白的cDNA先前克隆从鱼雷califonica使用抗血清纯化的突触囊泡。在这里，我们表明，该基因杂交到几个克隆在大鼠脑cDNA文库。这些克隆代表编码不同蛋白质的cDNA，从而确定了一个家族。这些蛋白质显示出与鱼雷蛋白相似的组织。同源性存在于7个氨基酸的重复内。如Southern分析以及序列分析所示，通过选择性剪接产生多样性。这些蛋白质在大鼠脑中特异性表达。原位杂交显示突触核蛋白mRNA在脑的离散区域中表达，海马是染色最强烈的区域。这种定位使人想起参与磷酸肌醇第二信使途径的那些分子。突触核蛋白以双重形式存在于大鼠脑中-一种可溶的小形式和一种以受调节的方式与突触体膜相关的大形式。
The synuclein cDNA was previously cloned from Torpedo califonica using an antiserum against purified synaptic vesicles. Here we show that this gene hybridizes to several clones in a rat brain cDNA library. These clones represent cDNAs coding for different proteins, thus determining a family. These proteins display similar organization to the Torpedo protein. The homology resides within the repetition of 7 amino acids. The diversity is generated by alternative splicing as suggested by Southern analysis, as well as by sequence analysis. These proteins are specifically expressed in the rat brain. In situ hybridization shows expression of synuclein mRNAs in discrete areas of the brain, the hippocampus is the most intensely stained area. This localization is reminiscent of those molecules involved in phosphoinositols second messenger pathways. Synuclein proteins are present in rat brain in dual form - a small form which is soluble and a large form which is associated with synaptosomal membranes in a regulated fashion.