Molecular and serological survey of hepatitis E virus infection among domestic pigs in Inner Mongolia, China
Molecular and serological survey of hepatitis E virus infection among domestic pigs in Inner Mongolia, China
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DOI：
10.1007/s00705-010-0706-0
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Jinshan;Jirintai;Okamoto, Hiroaki
为了解内蒙古地区猪戊型肝炎病毒（HEV）感染的流行情况和特点，对内蒙古地区14个猪场356头2 ~ 4月龄猪血清进行了HEV抗体和HEV RNA检测。总体而言，186头猪（52%）检测出抗HEV抗体阳性，而30头猪（8%）检测出HEV RNA水平。从病毒血症猪中回收的30株戊型肝炎病毒分离株在遗传学上被分类为基因型4，并且在ORF 2内的412 nt序列中彼此差异高达15.3%。内蒙古猪HEV株与中国其他省份分离的人或猪HEV株最相似，但与蒙古（以前称为外蒙古）的差异为15.9-18.9%。这些结果表明，在内蒙古的农场猪经常感染显着不同的基因型4戊型肝炎病毒株，可能是中国本土。
To evaluate the prevalence and characteristics of swine hepatitis E virus (HEV) infection in Inner Mongolia, China, serum samples obtained from 356 2- to 4-month-old pigs on 14 farms in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 186 pigs (52%) tested positive for anti-HEV antibodies, while 30 pigs (8%) had detectable HEV RNA levels. The 30 HEV isolates recovered from the viremic pigs were phylogenetically classified into genotype 4 and differed from each other by up to 15.3% in a 412 nt sequence within ORF2. The Inner Mongolian swine HEV strains were most similar to human or swine HEV strains isolated in the other provinces of China but differed by 15.9-18.9% from those in Mongolia (formerly known as Outer Mongolia). These results indicate that farm pigs in Inner Mongolia are frequently infected with markedly divergent genotype 4 HEV strains that may be indigenous to China.