Fish oil-based lipid emulsion alleviates parenteral nutrition-associated liver diseases and intestinal injury in piglets
Fish oil-based lipid emulsion alleviates parenteral nutrition-associated liver diseases and intestinal injury in piglets
复制标题
鱼油基脂肪乳剂可减轻仔猪肠外营养相关的肝脏疾病和肠道损伤。
DOI：
10.1002/jpen.2229
复制
发表时间：
2021-09-06
影响因子：
3.4
通讯作者：
Wang, Ying
中科院分区：
文献类型：
--
作者：
Chen, Shanshan;Xiao, Yongtao;Wang, Ying
本研究旨在研究鱼油基脂乳（FO）对肠外营养仔猪肠肝损伤和肠道微生物群的影响。方法将新生仔猪分为3组，分别为肠内饲粮（对照组）、添加100%鱼油的PN组和添加中链甘油三酯/长链甘油三酯脂乳（MCT/LCT）的PN组，饲喂14 d。测定血清生化指标、肝脏和肠道组织学以及炎症、氧化应激和脂质代谢相关基因的表达。分析血清胆汁酸（BA）谱和不同肠段肠道微生物组的分类组成。结果与MCT/ lct仔猪相比，鱼油可减轻肝脏炎症，促进脂肪酸氧化，降低氧化应激。在肠道中，与MCT/ lct -仔猪相比，FO降低了肠道炎症和肠道通透性，导致进入血液循环的脂多糖减少。PN组以变形菌门和拟杆菌门为主，对照组以厚壁菌门为主。FO改变了不同肠道菌群的分类组成，增加了回肠Bacteroidaceae以及结肠Rikenellaceae和Ruminococcaceae的相对丰度。鱼油处理改变了血清中BA的组成比例，降低了继发BA与原发性BA的比例。结论鱼油通过调节ba肠肝循环稳态和改变不同肠段微生物群组成，减轻PNLAD和肠道损伤。
Background Thisstudy aimed to investigate the impact of fish oil-based lipid emulsion (FO) on enterohepatic injuries and intestinal microbiota in piglets of parenteral nutrition (PN). Methods Newborn piglets were divided into three groups, including enteral diet (the controls), PN with 100% FO and PN with medium-chain triglyceride/long-chain triglyceride-based lipid emulsion (MCT/LCT) for 14 days. Serum biochemical indicators, hepatic and intestinal histology, and expression of genes associated with inflammation, oxidative stress, and lipid metabolism were measured. The bile acid (BA) profiles in serum and the taxonomic composition of the gut microbiome in different intestinal segments were analyzed. Results Compared with MCT/LCT-piglets, FO reduced inflammation, promoted fatty acid oxidation, and decreased oxidative stress in the liver. In the intestine, FO decreased intestinal inflammation and intestinal permeability, leading to reduced lipopolysaccharide entry into the blood circulation relative to MCT/LCT-piglets. PN groups have dominant contents of Proteobacteria and Bacteroides, whereas the control group have Firmicutes at the phylum level. FO altered the taxonomic compositions of the gut microbiome in different segments, increased the relative abundance of Bacteroidaceae in ileum, and Rikenellaceae and Ruminococcaceae in the colon. FO treatment shifted BA composition ratio in serum and had a lower ratio of secondary BAs to primary BAs. Conclusion FO alleviates PNLAD and intestinal injury by regulating the homeostasis of BAs' enterohepatic circulation and altering microbiota composition in different intestinal segments.