Effects of Dietary Protein to Carbohydrate Balance on Energy Intake, Fat Storage, and Heat Production in Mice
Effects of Dietary Protein to Carbohydrate Balance on Energy Intake, Fat Storage, and Heat Production in Mice
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DOI：
10.1002/oby.20007
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发表时间：
2013-01-01
期刊：
影响因子：
6.9
通讯作者：
Simpson, Stephen J.
中科院分区：
文献类型：
--
作者：
Huang, Xin;Hancock, Dale P.;Simpson, Stephen J.
目的：蛋白质杠杆在增加能量摄入方面发挥作用，这可能会促进体重增加。为了研究饲料中蛋白质与碳水化合物的比例(P：C)对C57BL/6J小鼠总能量摄入、脂肪储存和产热的影响。设计与方法：9周龄雄性小鼠随机选择5种P：C不同的等热量饮食之一，记录12周的食物摄入量。16周后，解剖白色脂肪组织(WAT)和棕色脂肪组织(BAT)沉积，称重，并测定BAT中关键代谢调节因子的表达水平。在另一个队列中，对25种蛋白质、脂肪和碳水化合物含量不同的饮食进行体表温度测量。结果：低磷：碳水化合物饮食(9：72和17：)的小鼠总能量摄入更多，WAT和蝙蝠储存增加。体表温度随着总能量摄入量以及蛋白质、脂肪和碳水化合物的增加而增加，对每千焦耳摄入量的贡献相似。在最低P：C饮食条件下，小鼠BAT中三种关键的产热调节因子的表达下调。结论：低蛋白饮食导致持续的吞噬功能亢进和脂肪储备的普遍扩张。低P：C饮食的体表温度升高与饮食诱导生热(DIT)是一致的，作为一种在此类饮食中分散过量摄入能量的手段，尽管这不足以防止肥胖症的增加。目前尚不清楚英美烟草是否参与了DIT。低P：C饮食中蝙蝠体重的增加可能说明了这一点，但产热基因表达模式并不支持BAT在DIT中的作用，尽管它们可能反映了长期暴露于低P：C饮食中导致产热功能的失败。
Objective: Protein leverage plays a role in driving increased energy intakes that may promote weight gain. The influence of the protein to carbohydrate ratio (P:C) in diets of C57BL/6J mice on total energy intake, fat storage, and thermogenesis was investigated.Design and Methods: Male mice (9 weeks old) were provided ad libitum access to one of five isocaloric diets that differed in P:C. Food intake was recorded for 12 weeks. After 16 weeks, white adipose tissue (WAT) and brown adipose tissue (BAT) deposits were dissected, weighed, and the expression levels of key metabolic regulators were determined in BAT. In a separate cohort, body surface temperature was measured in response to 25 diets differing in protein, fat, and carbohydrate content.Results: Mice on low P:C diets (9:72 and 17:64) had greater total energy intake and increased WAT and BAT stores. Body surface temperature increased with total energy intake and with protein, fat, and carbohydrate, making similar contributions per kJ ingested. Expression of three key regulators of thermogenesis were downregulated in BAT in mice on the lowest P:C diet.Conclusions: Low-protein diets induced sustained hyperphagia and a generalized expansion of fat stores. Increased body surface temperature on low P:C diets was consistent with diet-induced thermogenesis (DIT) as a means to dissipate excess ingested energy on such diets, although this was not sufficient to prevent development of increased adiposity. Whether BAT was involved in DIT is not clear. Increased BAT mass on low P:C diets might suggest so, but patterns of thermogenic gene expression do not support a role for BAT in DIT, although they might reflect failure of thermogenic function with prolonged exposure to a low P:C diet.