Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs
Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs
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DOI：
10.1007/s11103-006-9040-z
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发表时间：
2006-11-01
影响因子：
5.1
通讯作者：
Zhang, Qifa
中科院分区：
文献类型：
--
作者：
Huang, Yi;Zhang, Lida;Zhang, Qifa
尽管利用杂种优势对世界范围内的作物生产力做出了重大贡献，但杂种优势的生物学机制在很大程度上仍不清楚。本研究利用由9198个表达序列标签(ESTs)组成的基因芯片，分析了水稻优良杂交种及其亲本幼穗发育三个时期的基因表达谱，旨在揭示可能与产量杂种优势相关的基因表达模式。共有8422个序列在至少一个阶段显示了所有三种基因型的杂交信号，5771个序列在所有载片中都产生了可检测到的信号。在P<；0.05的100个排列中，经方差分析验证，在三个阶段中的至少一个阶段，三种基因类型中的438个序列的表达水平存在显著差异。141个序列表现出显著的中亲优势，表现出以下特点：负优势序列数量远多于正优势序列；参与DNA复制和修复的基因倾向于正优势；糖代谢、脂肪代谢、能量代谢、翻译、蛋白质降解和细胞信息处理等基因表现负优势；氨基酸代谢、转录、信号转导、植物防御和运输等基因均表现出正、负优势。这一结果表明，相对于双亲而言，杂种中发生的生化和生理活动。鉴定不同类型间表现出表达多态性的基因及其在杂种中的优势表达，可能为探索杂种优势的生物学机制提供新的途径。
Despite the significant contributions of utilizing heterosis to crop productivity worldwide, the biological mechanisms of heterosis remained largely uncharacterized. In this study, we analyzed gene expression profiles of an elite rice hybrid and the parents at three stages of young panicle development, using a cDNA microarray consisting of 9198 expressed sequence tags (ESTs), with the objective to reveal patterns of gene expression that may be associated with heterosis in yield. A total of 8422 sequences showed hybridization signals in all three genotypes in at least one stage and 5771 sequences produced detectable signals in all slides. Significant differences in expression level were detected for 438 sequences among the three genotypes in at least one of the three stages, as determined by ANOVA validated with 100 permutations at P < 0.05. Significant mid-parent heterosis was detected for 141 sequences, which demonstrated the following features: a much larger number of sequences showed negative heterosis than ones showing positive heterosis; genes functioning in DNA replication and repair tended to show positive heterosis; genes functioning in carbohydrate metabolism, lipid metabolism, energy metabolism, translation, protein degradation, and cellular information processing showed negative heterosis; both positive and negative heterosis were observed for genes in amino acid metabolism, transcription, signal transduction, plant defense and transportation. The results are indicative of the biochemical and physiological activities taking place in the hybrid relative to the parents. Identification of genes showing expression polymorphisms among different genotypes and heterotic expression in the hybrid may provide new avenues for exploring the biological mechanisms underlying heterosis.