Natural organic matter–induced alleviation of the phytotoxicity to rice (Oryza sativa L.) caused by copper oxide nanoparticles
Natural organic matter–induced alleviation of the phytotoxicity to rice (Oryza sativa L.) caused by copper oxide nanoparticles
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DOI：
10.1002/etc.3016
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发表时间：
2015-09
影响因子：
4.1
通讯作者：
Cheng Peng;H. Zhang;Huaxiang Fang;Chen Xu;Haomin Huang;Yi Wang;Lijuan Sun;Xiaofeng Yuan
中科院分区：
文献类型：
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作者：
Cheng Peng;H. Zhang;Huaxiang Fang;Chen Xu;Haomin Huang;Yi Wang;Lijuan Sun;Xiaofeng Yuan
天然有机物（NOM）可以与环境中的工程纳米颗粒（NPs）相互作用，并改变它们的行为和对生物体的毒性。在本研究中，氧化铜（CuO）纳米颗粒对水稻幼苗的植物毒性在腐殖酸作为NOM模型的存在下进行了研究。结果表明，纳米氧化铜处理抑制了根的伸长，导致根的形态和超微结构发生畸变，细胞活力和膜完整性丧失。CuO纳米粒子引起水稻幼苗体内活性氧的产生，导致膜脂过氧化、线粒体功能障碍和细胞程序性死亡。然而，通过添加腐殖酸减轻了所有植物毒性，因为纳米颗粒表面上的腐殖酸涂层增强了CuO NP与植物细胞壁/膜之间的静电和空间排斥，减少了NP与植物之间的接触以及CuO NP诱导的对植物细胞的氧化损伤。本研究的结果揭示了NP植物毒性的机制，并突出了NOM对NP生物利用度和毒性的影响。环境毒理化学2015;34：1996-2003。© 2015 SETAC
Natural organic matter (NOM) can interact with engineered nanoparticles (NPs) in the environment and modify their behavior and toxicity to organisms. In the present study, the phytotoxicity of copper oxide (CuO) NPs to rice seedlings in the presence of humic acid as a model NOM was investigated. The results showed that CuO NPs induced the inhibition of root elongation, aberrations in root morphology and ultrastructure, and losses of cell viability and membrane integrity. The adverse effects partly resulted from the generation of reactive oxygen species caused by CuO NPs, which led to lipid peroxidation, mitochondrial dysfunction, and programmed cell death in rice seedlings. However, all the phytotoxicity was alleviated with the addition of humic acid because humic acid coatings on nanoparticle surfaces enhanced electrostatic and steric repulsion between the CuO NPs and the plant cell wall/membrane, reducing contact between NPs and plant and CuO NP‐induced oxidative damage to plant cells. The present study's results shed light on the mechanism underlying NP phytotoxicity and highlight the influence of NOM on the bioavailability and toxicity of NPs. Environ Toxicol Chem 2015;34:1996–2003. © 2015 SETAC