Potential of sediment bacterial communities from Manila Bay (Philippines) to degrade low-density polyethylene (LDPE)
Potential of sediment bacterial communities from Manila Bay (Philippines) to degrade low-density polyethylene (LDPE)
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DOI：
10.1007/s00203-022-03366-y
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发表时间：
2022-12
影响因子：
2.8
通讯作者：
N. C. Gomez;D. F. Onda
中科院分区：
文献类型：
--
作者：
N. C. Gomez;D. F. Onda
塑料的持久性及其在不同环境中积累的影响，特别是在沿海地区，令人严重关切。这些塑料表现出降解迹象，可能是由微生物介导的。在这项研究中，我们调查了菲律宾马尼拉湾，这是一个严重的塑料问题，沉积物微生物群落降解低密度聚乙烯（LDPE）的潜力。对选定地点的塑料进行了量化，并从塑料积累最低和最高的地点收集了沉积物样本。然后引入这些沉淀物，并在体外与LDPE一起孵育91天。傅立叶变换红外光谱检测到的外观羰基和乙烯基产品的塑料表面上，表明结构表面改性归因于聚合物降解。使用16 S rRNA基因的V4-V5区域的高通量测序来分析附着于塑料的群落。变形菌门的成员在整个实验过程中占主导地位的塑料表面。一些细菌类群与烃类降解也丰富，与生物降解指数呈正相关的一些类群，这表明在塑料的部分生物降解的潜在积极作用。还存在其他分类群，它们可能消耗副产品或为其他群体提供营养，这表明利用塑料作为主要碳源和在塑料生物膜内创造微环境的协同作用。这项研究表明，来自马尼拉湾的沉积物微生物可能具有天然存在的微生物群体，可能能够部分降解塑料，支持先前的研究，即塑料的生物降解潜力普遍存在于海洋微生物组合中。
The persistence of plastics and its effects in different environments where they accumulate, particularly in coastal areas, is of serious concern. These plastics exhibit signs of degradation, possibly mediated by microorganisms. In this study, we investigated the potential of sediment microbial communities from Manila Bay, Philippines, which has a severe plastics problem, to degrade low-density polyethylene (LDPE). Plastics in selected sites were quantified and sediment samples from sites with the lowest and highest plastic accumulation were collected. These sediments were then introduced and incubated with LDPE in vitro for a period of 91 days. Fourier transform infrared spectroscopy detected the appearance of carbonyl and vinyl products on the plastic surface, indicating structural surface modifications attributed to polymer degradation. Communities attached to the plastics were profiled using high-throughput sequencing of the V4-V5 region of the 16S rRNA gene. Members of the phylum Proteobacteria dominated the plastic surface throughout the experiment. Several bacterial taxa associated with hydrocarbon degradation were also enriched, with some taxa positively correlating with the biodegradation indices, suggesting potential active roles in the partial biodegradation of plastics. Other taxa were also present, which might be consuming by-products or providing nourishment for other groups, indicating synergy in utilizing the plastic as the main carbon source and creation of a microenvironment within the plastics biofilm. This study showed that sediment microbes from Manila Bay may have naturally occurring microbial groups potentially capable of partially degrading plastics, supporting previous studies that the biodegradation potential for plastics is ubiquitously present in marine microbial assemblages.