Blood alcohol concentrations after scheduled access in high-alcohol-preferring mice
Blood alcohol concentrations after scheduled access in high-alcohol-preferring mice
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DOI：
10.1016/j.alcohol.2003.08.002
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发表时间：
2003-08-01
期刊：
影响因子：
2.3
通讯作者：
Grose, AM
中科院分区：
文献类型：
--
作者：
Grahame, NJ;Grose, AM
开发程序，产生大量的血液酒精浓度在自愿访问的酒精溶液中的小鼠是必要的，以进一步表征遗传和神经生物学机制的酒精自我管理。虽然在实验情况下，一些小鼠群体很容易饮用酒精溶液，但以前的研究结果通常没有显示自愿进入后血液中的酒精浓度较高，这可能是因为小鼠的酒精代谢率较高。为了建立小鼠饮酒模型，对36只选择性繁殖的高酒精偏好小鼠进行了30分钟的预定访问程序，通过使用糖精褪色逐渐引入酒精溶液。小鼠每天24小时可随意获取食物和水。在周期的黑暗部分开始后1小时，可获得酒精溶液，每天30分钟，每周5天。从饮水管中完全除去糖精后，小鼠持续饮用1.4g/kg的10% [体积/体积（vol./卷）]在30分钟进入期结束后立即采集的尾血样品的分析表明，血液酒精浓度与酒精摄入量密切相关（范围，6-130 mg/dl;平均值，接近60 mg/dl）。10%、12%、15%、18%和21%（vol./卷）酒精溶液表明酒精摄入量和酒精浓度之间呈倒U形关系，当可获得15%酒精溶液时，每30分钟的峰值摄入量大于1.75 g/kg。未观察到性别差异。这些发现表明，该程序在获得自愿口服酒精溶液后小鼠的酒精相关血液浓度中的实用性。(C)2003年爱思唯尔公司All rights reserved.
Development of procedures yielding substantial blood alcohol concentrations during voluntary access to an alcohol solution in mice is necessary to further characterize genetic and neurobiologic mechanisms underlying alcohol self-administration. Although, in experimental situations, some populations of mice readily drink an alcohol solution, results from previous studies have not typically revealed high blood alcohol concentrations after voluntary access, probably because of the high alcohol metabolism rate in mice. Toward development of a murine drinking model, 36 selectively bred high-alcohol-preferring mice of both sexes were subjected to a 30-min scheduled-access procedure by using saccharin fading to gradually introduce an alcohol solution. Mice had ad libitum access to food and water 24 h a day. The alcohol solution was available I h after the start of the dark part of the cycle for 30 min per day, 5 days per week. After complete removal of saccharin from the drinking tubes, mice consistently drank 1.4 g/kg of a 10% [volume/volume (vol./vol.)] alcohol solution in 30 min. Analysis of tail blood samples, taken immediately after the end of the 30-min access period, indicated blood alcohol concentrations were tightly correlated with alcohol intakes (range, 6-130 mg/dl; average, nearly 60 mg/dl). A concentration-response function of 10%, 12%, 15%, 18%, and 21% (vol./vol.) alcohol solutions indicated an inverted U-shaped relation between alcohol intake and alcohol concentration, with peak intake of greater than 1.75 g/kg per 30 min when a 15% alcohol solution was available. No sex differences were seen. These findings indicate the utility of this procedure in obtaining pharmacologically relevant blood alcohol concentrations after voluntary oral self-administration of an alcohol solution in mice. (C) 2003 Elsevier Inc. All rights reserved.