Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum.
Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum.
复制标题
磷脂酶 cbeta4 特别参与发育中小脑中攀爬纤维突触的消除。
DOI：
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发表时间：
1998
影响因子：
11.1
通讯作者：
Dianqing Wu
中科院分区：
文献类型：
--
作者：
Masanobu Kano;K. Hashimoto;Masahiko Watanabe;H. Kurihara;Stefan Offermanns;Huiping Jiang;Yanping Wu;Kisun Jun;Hee;Y. Inoue;Melvin I. Simon;Dianqing Wu
小脑发育过程中过量攀爬纤维(CF)-浦肯野细胞突触的消除涉及一个信号通路，该信号通路包括1型代谢性谷氨酸受体、Galphaq和蛋白激酶C的γ亚型。为了鉴定参与这一过程的磷脂酶C （PLC）亚型，我们制造了浦肯野细胞中表达的两种主要亚型之一PLCbeta4缺失的小鼠。PLCbeta4突变小鼠存活，但表现出运动性共济失调。他们的小脑组织学、平行纤维突触的形成和基本电生理表现正常。然而，多发性CF神经支配的发育消除在小脑蚓喙部明显受损，其中PLCbeta4 mRNA主要表达。相比之下，CF突触消除在尾侧小脑中是正常的，其中PLCbeta4 mRNA水平低，而PLCbeta3 mRNA水平高。这些结果表明，PLCbeta4转导的信号是小脑吻侧CF突触消除所必需的。
Elimination of excess climbing fiber (CF)-Purkinje cell synapses during cerebellar development involves a signaling pathway that includes type 1 metabotropic glutamate receptor, Galphaq, and the gamma isoform of protein kinase C. To identify phospholipase C (PLC) isoforms involved in this process, we generated mice deficient in PLCbeta4, one of two major isoforms expressed in Purkinje cells. PLCbeta4 mutant mice are viable but exhibit locomotor ataxia. Their cerebellar histology, parallel fiber synapse formation, and basic electrophysiology appear normal. However, developmental elimination of multiple CF innervation clearly is impaired in the rostral portion of the cerebellar vermis, in which PLCbeta4 mRNA is predominantly expressed. By contrast, CF synapse elimination is normal in the caudal cerebellum, in which low levels of PLCbeta4 mRNA but reciprocally high levels of PLCbeta3 mRNA are found. These results indicate that PLCbeta4 transduces signals that are required for CF synapse elimination in the rostral cerebellum.
DOI：
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发表时间：
1993-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Hepler;T. Kozasa;A. Smrcka;Melvin I. Simon;S. Rhee;P. Sternweis;A. Gilman
通讯作者：
J. Hepler;T. Kozasa;A. Smrcka;Melvin I. Simon;S. Rhee;P. Sternweis;A. Gilman
DOI：
10.1073/pnas.87.7.2662
发表时间：
1990-04-01
影响因子：
11.1
作者：
KONNERTH, A;LLANO, I;ARMSTRONG, CM
通讯作者：
ARMSTRONG, CM