Lysis of human solid tumors by autologous cells sensitized in vitro to alloantigens.
Lysis of human solid tumors by autologous cells sensitized in vitro to alloantigens.
复制标题
通过体外对同种异体抗原敏感的自体细胞裂解人实体瘤。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
S. A. Rosenberg
中科院分区：
文献类型：
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作者：
J. Strausser;Amitabha Mazumder;E. A. Grimm;M. T. Lotze;S. A. Rosenberg
评价了体外对同种异体单供体细胞以及同种异体供体细胞池致敏的人淋巴细胞对自体新鲜实体瘤细胞、自体组织培养的肿瘤和成纤维细胞、新鲜外周血淋巴样细胞（PBL）和PBL的Con A母细胞的细胞毒性潜力。使用4小时51铬释放试验，池致敏效应细胞裂解自体组织培养的肿瘤和成纤维细胞的目标在4个实验。类似地，在使用单供体同种异体致敏效应物的2个实验中，自体组织培养的肿瘤被裂解。未观察到新鲜PBL溶解。在12个使用池致敏效应物的实验中的10个中观察到新鲜自体肿瘤靶细胞的显著溶解，尽管再次没有观察到自体PBL的溶解。在2/2个实验中未观察到自体Con A母细胞溶解。新鲜的肿瘤细胞，PBL，和Con A母细胞似乎同样裂解，因为它们同样裂解的效应细胞特异性致敏的同种异体抗原存在于这些细胞。冷靶抑制研究表明，培养的肿瘤和成纤维细胞，但不是PBL共享池致敏细胞识别的决定因素。
Human lymphocytes sensitized in vitro to allogeneic single-donor cells as well as to pools of allogeneic donor cells were evaluated for their cytotoxic potential against autologous fresh solid tumor cells, autologous tissue-cultured tumor and fibroblasts, fresh peripheral blood lymphoid cells (PBL), and Con A blasts of PBL. Using 4-hr 51Cr release assays, pool-sensitized effector cells lysed autologous tissue-cultured tumor and fibroblast targets in 4 of 4 experiments. Similarly, in 2 of 2 experiments with single-donor allosensitized effectors, autologous tissue-cultured tumor was lysed. No lysis of fresh PBL was seen. Significant lysis of fresh autologous tumor target cells was seen in 10 of 12 experiments with pool-sensitized effectors, though, again, no lysis of autologous PBL was observed. No lysis of autologous Con A blasts was seen in 2 of 2 experiments. Fresh tumor cells, PBL, and Con A blasts appeared equally lysable, since they were equally lysed by effector cells specifically sensitized to alloantigens present on these cells. Cold target inhibition studies demonstrated that cultured tumor and fibroblasts but not PBL shared the determinants recognized by pool-sensitized cells.