Genetic mapping of pathogenicity and aggressiveness of Gibberella zeae (Fusarium graminearum) toward wheat
Genetic mapping of pathogenicity and aggressiveness of Gibberella zeae (Fusarium graminearum) toward wheat
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DOI：
10.1094/phyto.2004.94.5.520
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发表时间：
2004-05-01
期刊：
影响因子：
3.2
通讯作者：
Miedaner, T
中科院分区：
文献类型：
--
作者：
Cumagun, CJR;Bowden, RL;Miedaner, T
小麦赤霉病菌是小麦赤霉病的主要病原真菌，产生多种毒素，对人类和家养一年生植物都有危害。我们在一张基于扩增片段长度多态性的遗传图谱上鉴定了与致病性和攻击性相关的位点。来自日本的谱系6雪腐镰刀菌烯醇生产者和来自堪萨斯的谱系7脱氧雪腐镰刀菌烯醇生产者之间的杂交。在温室中对99个后代和亲本进行了2年的测试。后代分离的致病性和非致病性，分别定性（61：38）。该性状映射到连锁群IV，该连锁群邻近影响菌落色素沉着、囊壳产生和紫球藻毒素量的基因座。在61个致病性后代中，诱导的疾病量（攻击性）在数量上变化。利用简单区间分析在连锁群1上检测到两个攻击性QTL。一个与TR15基因座（在二萜途径基因簇中的二萜合酶）连锁的QTL解释了观察到的51%的变异，而另一个距离50厘摩的QTL解释了29%的表型变异。TR15紧密连锁于控制蓖麻毒素类型的基因座。然而，使用复合区间分析，这两个QTL可能是同一QTL的一部分。平均而言，产生脱氧雪腐镰刀菌烯醇的后代大约是产生雪腐镰刀菌烯醇的后代的两倍。没有发现攻击性的超亲分离。这两个性状在此系间杂交中的相当简单的遗传表明，相对较少的致病性或侵略性位点在谱系6和7之间不同。
Gibberella zeae is the major fungal pathogen of Fusarium head blight of wheat and produces several mycotoxins that are harmful to humans and domesticated annuals. We identified loci associated with pathogenicity and aggressiveness on an amplified fragment length polymorphism based genetic map of G. zeae in a cross between a lineage 6 nivalenol producer from Japan and a lineage 7 deoxynivalenol producer from Kansas. Ninety-nine progeny and the parents were tested in the greenhouse for 2 years. Progeny segregated qualitatively (61:38) for pathogenicity:nonpathogenicity, respectively. The trait maps to linkage group IV, which is adjacent to loci that affect colony pigmentation, perithecium production, and trichothecene toxin amount. Among the 61 pathogenic progeny, the amount of disease induced (aggressiveness) varied quantitatively. Two reproducible quantitative trait loci (QTL) for aggressiveness were detected on linkage group 1 using simple interval analysis. A QTL linked to the TR15 locus (trichodiene synthase in the trichothecene pathway gene cluster) explained 51% of the variation observed, and a second QTL that was 50 centimorgans away explained 29% of the phenotypic variation. TR15 is tightly linked to the locus controlling trichothecene toxin type. The two QTLs, however, were likely part of the same QTL using composite interval analysis. Progeny that produced deoxynivalenol were, on average, approximately twice as aggressive as those that produced nivalenol. No transgressive segregation for aggressiveness was detected. The rather simple inheritance of both traits in this interlineage cross suggests that relatively few loci for pathogenicity or aggressiveness differ between lineage 6 and 7.