VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro
VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro
复制标题
VEGF165 通过体外激活 VEGFR-2 调节中央毛囊上皮培养的人外根鞘细胞的增殖、粘附、迁移和分化。
DOI：
10.1016/j.jdermsci.2013.10.002
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发表时间：
2014-02-01
影响因子：
4.6
通讯作者：
Lu Zhong-Fa
中科院分区：
文献类型：
--
作者：
Wu Xian-Jie;Zhu Jian-Wei;Lu Zhong-Fa
Background: The functional state of vasculature is tightly controlled by vascular endothelial growth factor receptor-2 (VEGFR-2). Recent studies revealed that VEGFR-2 is expressed on hair follicle keratinocytes.Objective: We proposed to investigate its effect on proliferation, adhesion and migration of cultured human outer root sheath cells from central hair follicle epithelium.Methods: These studies were undertaken in vitro using human outer root sheath cells from central hair follicle epithelium, immunohistochemistry analysis, immunofluorescence microscopy, western blot analysis, MU, trans well analysis, and RT-PCR.Results: Our results show that VEGFR-2 is expressed in these cells in vivo and in vitro. Furthermore, proliferation and migration of cultured human outer root sheath cells from central hair follicle epithelium is increased by VEGF(165), while homotypic adhesion is decreased but heterotypic adhesion is increased. VEGF(165) upregulates integrin beta 1 but dowregulates Igr6 expression. In addition, phosphorylation of VEGFR-2, Erk1/2, c-Jun and p38, are increased following VEGF(165) treatment and these effects are reversed by a VEGFR-2 neutralizing antibody.Conclusion: Our results suggest a role of VEGF/VEGFR-2 beyond angiogenesis in hair follicle regulation. (C) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
