Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major
Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major
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DOI：
10.1111/j.1365-2583.2004.00539.x
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发表时间：
2005-04-01
影响因子：
2.6
通讯作者：
Lanzaro, GC
中科院分区：
文献类型：
--
作者：
Elnaiem, DEA;Meneses, C;Lanzaro, GC
SP-15 是一种白蛉唾液蛋白，可针对由大型利什曼原虫引起的皮肤利什曼病提供强有力的保护，并已被提议作为针对这种疾病的潜在疫苗。为了研究该蛋白可能存在的抗原变异，我们检测了 100 只白蛉 (Phlebotomus papatasi) 中 SP-15 的遗传多态性，这些白蛉来自苏丹的自然群体以及埃及、约旦、以色列和沙特阿拉伯的四个实验室群体。我们发现，虽然在自然界中可能发现 SP-15 的许多变体，但它们之间的差异很小（对于 40 个核苷酸序列，平均+/- SD 成对差异 = 1.69 +/- 0.83%；对于 30 个氨基酸序列变体，平均 +/- SD 成对差异 = 3.06 +/- 1.13%）。同义和非同义取代比例分析表明SP-15没有受到多样化选择。我们的结果表明，基于 SP-15 蛋白的疫苗应该会产生一致的免疫反应。
SP-15 is a sandfly salivary protein that provides strong protection against cutaneous leishmaniasis, caused by Leishmania major, and has been proposed as a potential vaccine against this disease. To investigate possible antigenic variation in this protein, we examined genetic polymorphism of SP-15 in 100 Phlebotomus papatasi sandflies, from a natural population from Sudan and four laboratory colonies from Egypt, Jordan, Israel and Saudi Arabia. We found that although many variants of SP-15 may be found in nature, differences among them are minimal (mean +/- SD pairwise differences = 1.69 +/- 0.83% for forty nucleotide sequences and 3.06 +/- 1.13% for thirty amino acid sequence variants). Analysis of proportions of synonymous and non-synonymous substitutions indicated that SP-15 is not under diversifying selection. Our results suggest that a vaccine based on SP-15 protein should result in a uniform immune response.