kappa+lambda+ dual receptor B cells are present in the human peripheral repertoire.
kappa+lambda+ dual receptor B cells are present in the human peripheral repertoire.
复制标题
Kappa+ lambda+双受体B细胞存在于人的外围曲目中。
DOI：
10.1084/jem.181.3.1245
复制
发表时间：
1995-03-01
影响因子：
15.3
通讯作者：
Lanzavecchia, Antonio
中科院分区：
文献类型：
--
作者：
Giachino, Claudia;Padovan, Elisabetta;Lanzavecchia, Antonio
成熟的B淋巴细胞表达κ或λ轻链（L）是一个普遍的概念，尽管导致这种同种型排斥的机制仍有争议。我们研究了正常人外周血B淋巴细胞L链同种型排斥的程度。通过用抗-CD 19、抗-κ和抗-λ抗体进行三色染色，我们可以估计0.2-0.5%的来自健康成人的外周血B细胞在细胞表面上表达κ和λ。对κ + λ +细胞进行分选，通过EB病毒使其永生化，并对5个独立克隆进行详细表征。所有克隆在细胞表面上表达κ和λ，并产生在同一分子中含有κ和λ链的免疫球蛋白M，即，杂交抗体L链的测序揭示了五分之三的克隆体细胞突变的证据。有趣的是，在一组单受体B细胞克隆中，我们鉴定出两个λ +克隆，它们携带有效重排的kappa，该kappa被体细胞突变产生的终止密码子灭活。这些结果表明，双受体B淋巴细胞中可以发现成熟的抗原选择的B细胞，并建议体细胞突变可以通过灭活乘客，非选择的L链，有助于增加同型排斥的程度。
It is a common notion that mature B lymphocytes express either kappa or lambda light (L) chains, although the mechanism that leads to such isotypic exclusion is still debated. We have investigated the extent of L chain isotypic exclusion in normal human peripheral blood B lymphocytes. By three-color staining with anti-CD19, anti-kappa, and anti-lambda antibodies we could estimate that 0.2-0.5% of peripheral blood B cells from healthy adults express both kappa and lambda on the cell surface. The kappa+lambda+ cells were sorted, immortalized by Epstein-Barr virus, and five independent clones were characterized in detail. All clones express both kappa and lambda on the cell surface and produce immunoglobulin M that contain both kappa and lambda chains in the same molecule, i.e., hybrid antibodies. Sequencing of the L chains revealed in three out of five clones evidence for somatic mutations. It is interesting to note that among a panel of single receptor B cell clones we identified two lambda+ clones that carried a productively rearranged kappa, which was inactivated by a stop codon generated by somatic mutation. These findings indicate that dual receptor B lymphocytes can be found among mature antigen-selected B cells and suggest that somatic mutation can contribute to increase the degree of isotypic exclusion by inactivating a passenger, nonselected L chain.
登录
查看更多内容
DOI：
10.1155/1990/56014
发表时间：
1990-01-01
期刊：
Developmental immunology
影响因子：
--
作者：
Berg, J;McDowell, M;Wabl, M
通讯作者：
Wabl, M
影响因子：
5.4
作者：
LAUTNERRIESKE, A;HUBER, C;ZACHAU, HG
通讯作者：
ZACHAU, HG
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N
DOI：
10.1084/jem.177.4.1165
发表时间：
1993-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Radic MZ;Erikson J;Litwin S;Weigert M
通讯作者：
Weigert M
DOI：
10.1084/jem.177.4.999
发表时间：
1993-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gay D;Saunders T;Camper S;Weigert M
通讯作者：
Weigert M