Transgenic technologies to induce sterility.
Transgenic technologies to induce sterility.
复制标题
DOI：
10.1186/1475-2875-8-s2-s7
复制
发表时间：
2009-11-16
期刊：
影响因子：
3
通讯作者：
Wimmer EA
中科院分区：
文献类型：
--
作者：
Catteruccia F;Crisanti A;Wimmer EA
在过去几年中，可用于对人类疟疾病媒按蚊的基因组进行分子和遗传研究的工具数量有了相当大的增加。因此，对蚊子生物学方面的知识，如与它们传播疾病的能力有关的免疫、繁殖和行为方面的知识正在迅速增加，这些知识可以转化为疟疾控制战略的具体利益。在最重要的科学进展中，针对按蚊的转基因技术的发展为改进现有的媒介控制措施或设计新的媒介控制措施提供了重要的机会。特别是，对蚊子基因组进行基因改造可以在野外更有效地部署昆虫不育技术（SIT）来对付病媒种群。目前，SIT依靠辐射绝育雄虫的释放，这些雄虫与野生雄虫竞争与野生雌虫交配。通过对蚊子基因组的遗传操纵诱导雄性不育，已经在许多其他昆虫物种中实现，可以消除对辐射的需要并提高基于sit的策略的效率。本文综述了目前用于果蝇和其他昆虫的转基因诱导不育的机制，并推测了将类似方法应用于按蚊的可能方法。
The last few years have witnessed a considerable expansion in the number of tools available to perform molecular and genetic studies on the genome of Anopheles mosquitoes, the vectors of human malaria. As a consequence, knowledge of aspects of the biology of mosquitoes, such as immunity, reproduction and behaviour, that are relevant to their ability to transmit disease is rapidly increasing, and could be translated into concrete benefits for malaria control strategies. Amongst the most important scientific advances, the development of transgenic technologies for Anopheles mosquitoes provides a crucial opportunity to improve current vector control measures or design novel ones. In particular, the use of genetic modification of the mosquito genome could provide for a more effective deployment of the sterile insect technique (SIT) against vector populations in the field. Currently, SIT relies on the release of radiation sterilized males, which compete with wild males for mating with wild females. The induction of sterility in males through the genetic manipulation of the mosquito genome, already achieved in a number of other insect species, could eliminate the need for radiation and increase the efficiency of SIT-based strategies. This paper provides an overview of the mechanisms already in use for inducing sterility by transgenesis in Drosophila and other insects, and speculates on possible ways to apply similar approaches to Anopheles mosquitoes.