Continuous particle separation through deterministic lateral displacement
Continuous particle separation through deterministic lateral displacement
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DOI：
10.1126/science.1094567
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发表时间：
2004-05-14
期刊：
影响因子：
56.9
通讯作者：
Sturm, JC
中科院分区：
文献类型：
--
作者：
Huang, LR;Cox, EC;Sturm, JC
我们报告了一种微流体颗粒分离装置，该装置利用障碍物周围层流的不对称分叉。粒子根据其大小确定性地选择其路径。所有给定尺寸的颗粒都遵循相同的迁移路径，从而获得高分辨率。用于表征该器件的 0.8、0.9 和 1.0 微米的微球在 40 秒内完成分选，分辨率接近 10 纳米，优于传统流动技术的时间和分辨率。细菌人工染色体可在10分钟内分离，分辨率约为12%。
We report on a microfluidic particle-separation device that makes use of the asymmetric bifurcation of laminar flow around obstacles. A particle chooses its path deterministically on the basis of its size. All particles of a given size follow equivalent migration paths, leading to high resolution. The microspheres of 0.8, 0.9, and 1.0 micrometers that were used to characterize the device were sorted in 40 seconds with a resolution of similar to10 nanometers, which was better than the time and resolution of conventional flow techniques. Bacterial artificial chromosomes could be separated in 10 minutes with a resolution of similar to12%.