Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer
Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer
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DOI：
10.1093/carcin/23.11.1927
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发表时间：
2002-11-01
期刊：
影响因子：
4.7
通讯作者：
Kanematsu, T
中科院分区：
文献类型：
--
作者：
Fukuda, K;Kuroki, T;Kanematsu, T
最近从鼠类的胆汁和肝胆系统中分离出几种螺杆菌，并被公认为是肝胆疾病的病原体。本研究旨在探讨幽门螺杆菌是否具有引起人类肝胆疾病，特别是肝胆癌的可能性。应用聚合酶链反应（PCR）技术对19例肝胆癌和19例良性胆道疾病患者的胆汁和肝胆组织标本进行了螺杆菌DNA的检测。使用增殖细胞核抗原（PCNA）染色技术，我们还研究了胆管上皮细胞动力学，特别是在肝胆系统中存在螺杆菌DNA。我们发现19例肝胆癌中10例（52.6%）螺杆菌DNA阳性。乳腺癌的发生率明显高于良性病变（15.7%）（P = 0.03）。无论患者是否患有肝胆癌和/或胆道炎症，螺杆菌DNA阳性患者的胆管上皮中的PCNA标记指数均高于螺杆菌DNA阴性患者，具有统计学意义。多元回归分析表明，幽门螺杆菌DNA的存在和胆管上皮中PCNA标记指数的升高之间存在密切的相关性。我们的研究结果表明，螺杆菌物种可能通过加速胆汁细胞动力学在肝胆癌的发病机制中发挥作用。
Several Helicobacter species have recently been isolated from the bile and hepatobiliary systems of murine species, and are well recognized as a pathogen of the hepatobiliary disorder. This study was planned to investigate whether Helicobacter species possess a causative potential for human hepatobiliary disease, especially for hepatobiliary carcinogenesis. Bile and hepatobiliary tissue samples from 19 patients with hepatobiliary cancer and 19 patients with benign biliary diseases were subjected to polymerase chain reaction analyses for the detection of Helicobacter DNAs. Using a proliferating cell nuclear antigen (PCNA) staining technique, we also investigated the biliary epithelial cell kinetics with special reference to the presence of Helicobacter DNAs in the hepatobiliary system. We found that Helicobacter DNAs were positive in 10 (52.6%) of the 19 patients with hepatobiliary cancer. The incidence was significantly higher than that (15.7%) in the benign cases (P = 0.03). The PCNA labeling index in the biliary epithelium in Helicobacter DNA-positive patients was statistically higher than that in Helicobacter DNA-negative ones, regardless of whether the patient was suffering from hepatobiliary cancer and/or biliary inflammation. A close correlation between the presence of Helicobacter DNAs and an elevation of the PCNA labeling index in the biliary epithelium was demonstrated by multiple regression analysis. Our findings suggest that Helicobacter species may play a role in the pathogenesis of hepatobiliary cancer through an acceleration of biliary cell kinetics.