A Segmentation Clock with Two-Segment Periodicity in Insects
A Segmentation Clock with Two-Segment Periodicity in Insects
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DOI：
10.1126/science.1218256
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发表时间：
2012-04-20
期刊：
影响因子：
56.9
通讯作者：
Averof, Michalis
中科院分区：
文献类型：
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作者：
Sarrazin, Andres F.;Peel, Andrew D.;Averof, Michalis
脊椎动物的分割依赖于一种以基因表达振荡为特征的机制。这种机制是否被其他节段动物使用一直存在争议。节肢动物分割过程中循环表达的严格证据一直缺乏。我们发现，在卡氏拟谷盗中，片段基因奇数跳跃(TC-ODD)以两段周期振荡。通过将胚胎一分为二并在不同的时间间隔内培养两半，我们证明了TC-ODD在30℃下的周期约为95分钟。使用绿色荧光蛋白表达胚胎的实时成像和细胞跟踪，我们可以排除细胞运动可以解释这种动态表达。我们的结果表明，分子振荡器代表了不同动物分割的共同特征，并有助于协调昆虫和脊椎动物分割的不同范式。
Vertebrate segmentation relies on a mechanism characterized by oscillating gene expression. Whether this mechanism is used by other segmented animals has been controversial. Rigorous proof of cyclic expression during arthropod segmentation has been lacking. We find that the segmentation gene odd-skipped (Tc-odd) oscillates with a two-segment periodicity in the beetle Tribolium castaneum. By bisecting embryos and culturing the two halves over different time intervals, we demonstrate that Tc-odd cycles with a period of about 95 minutes at 30 degrees C. Using live imaging and cell tracking in green fluorescent protein-expressing embryos, we can exclude that cell movements explain this dynamic expression. Our results show that molecular oscillators represent a common feature of segmentation in divergent animals and help reconcile the contrasting paradigms of insect and vertebrate segmentation.